Obtaining biologically active substances from Amaranthus caudatus L. seeds in one technological cycle

• Published in: Biomedical chromatography 2022-08, Vol.36 (8), p.e5386-n/a
• Main Authors: Korabel, Ivan M., Panchak, Lidiia V., Zyn, Alina R., Antonyuk, Volodymyr O.
• Format: Article
• Language: English
• Subjects: Amaranthus caudatus L; fatty oil; lectin; purification; squalene; triterpenoids
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.5386

Nowadays, amaranth is a valuable multipurpose crop and a source of a number of very important biologically active substances. The aim of this study was to develop a comprehensive scheme for obtaining fatty oil, triterpenoids and lectin from the seeds of Amaranthus caudatus L. in one technological cycle. Two variants of the lectin and triterpene compound purification method from amaranth seeds were tested. It was determined that the extraction of triterpene compounds should be carried out after purification of the lectin from degreased seeds. The rationality of this sequence of technological operations is explained by the lability of the lectin and the insolubility in water of triterpene compounds from amaranth seeds. The study also presents a scheme for obtaining squalene from amaranth oil by chromatography on silica gel and proposes a more effective affinity sorbent for purification of the lectin. The use of such a sorbent also opens up the possibility of preserving other water‐soluble substances from amaranth seeds. The physicochemical characteristics and carbohydrate specificity of the lectin are described and new data on the results of the interaction of lectin with human and animal erythrocytes are given. The obtained results are discussed in the light of the complex use of raw materials.