A modular microfluidic platform to enable complex and customisable in vitro models for neuroscience

• Published in: Lab on a chip 2022-05, Vol.22 (10), p.1989-2000
• Main Authors: Megarity, D, Vroman, R, Kriek, M, Downey, P, Bushell, T J, Zagnoni, M
• Format: Article
• Language: English
• Subjects: Central nervous system; Functional analysis; Microfluidics; Public health
• ISSN: 1473-0197; 1473-0189
• DOI: 10.1039/d2lc00115b

Disorders of the central nervous system (CNS) represent a global health challenge and an increased understanding of the CNS in both physiological and pathophysiological states is essential to tackle the problem. Modelling CNS conditions is difficult, as traditional models fail to recapitulate precise microenvironments and animal models of complex disease often have limited translational validity. Microfluidic and organ-on-chip technologies offer an opportunity to develop more physiologically relevant and complex models of the CNS. They can be developed to allow precise cellular patterning and enhanced experimental capabilities to study neuronal function and dysfunction. To improve ease-of-use of the technology and create new opportunities for novel studies, we introduce a modular platform consisting of multiple, individual microfluidic units that can be combined in several configurations to create bespoke culture environments. Here, we report proof-of-concept experiments creating complex models and performing functional analysis of neuronal activity across modular interfaces. This platform technology presents an opportunity to increase our understanding of CNS disease mechanisms and ultimately aid the development of novel therapies.