A flavonoid-rich fraction of Monolluma quadrangula inhibits xanthine oxidase and ameliorates potassium oxonate-induced hyperuricemia in rats

Hyperuricemia represents a risk factor for the progression of chronic kidney disease. Oxidative stress and inflammation are implicated in the mechanisms underlying hyperuricemia-mediated kidney injury. Monolluma quadrangula possesses several beneficial effects; however, its effect on hyperuricemia h...

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Published in:Environmental science and pollution research international 2022-09, Vol.29 (42), p.63520-63532
Main Authors: ALRashdi, Barakat M., Elgebaly, Hassan A., Germoush, Mousa O., Qarmush, Moath M., Azab, Mona S., Alruhaimi, Reem S., Ahmeda, Ahmad F., Abukhalil, Mohammad H., Kamel, Emadeldin M., Arab, Hany H., Alzoghaibi, Mohammed A., Alotaibi, Mohammed F., Mahmoud, Ayman M.
Format: Article
Language:English
Subjects:
Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Catalase
Creatinine
Cytokines
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental science
Flavonoids
Hyperuricemia
Inflammation
Kidney diseases
Kidneys
Molecular docking
Oxidative stress
Potassium
Research Article
Risk analysis
Risk factors
Scavenging
Urea
Uric acid
Waste Water Technology
Water Management
Water Pollution Control
Xanthine oxidase
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Summary:Hyperuricemia represents a risk factor for the progression of chronic kidney disease. Oxidative stress and inflammation are implicated in the mechanisms underlying hyperuricemia-mediated kidney injury. Monolluma quadrangula possesses several beneficial effects; however, its effect on hyperuricemia has not been investigated. This study evaluated the renoprotective and xanthine oxidase (XO) inhibitory activity of M. quadrangula in hyperuricemic rats. Phytochemical investigation revealed the presence of six known flavonoid isolated for the first time from this species. The rats received M. quadrangula extract (MQE) and potassium oxonate (PO) for 7 days. In vitro assays showed the radical scavenging and XO inhibitory activities of MQE, and in silico molecular docking revealed the inhibitory activity of the isolated flavonoids towards XO. Hyperuricemic rats showed elevated serum uric acid, creatinine, urea, and XO activity, and renal pro-inflammatory cytokines, MDA and NO, and decreased GSH, SOD, and catalase. MQE ameliorated serum uric acid, urea, creatinine, and XO activity, and renal pro-inflammatory cytokines. In addition, MQE attenuated renal oxidative stress, enhanced antioxidants, downregulated URAT-1, and GLUT-9 and upregulated OAT-1 in PO-induced rats. In conclusion, M. quadrangula attenuated hyperuricemia and kidney impairment by suppressing XO activity, oxidative stress and inflammation, and modulating urate transporters.
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-022-20274-2