Targeted gene next‐generation sequencing reveals genomic profile in a cohort of 46 Chinese patients with breast cancer

• Published in: The journal of gene medicine 2022-06, Vol.24 (6), p.e3420-n/a
• Main Authors: Yao, Jie, Chen, Qian, Zhu, Jun‐qi, Cai, Rui‐gang
• Format: Article
• Language: English
• Subjects: Breast cancer; Frameshift mutation; Gene therapy; Genes; Genomics; Hoxa11 gene; HOXA11 mutation; Lymphocytes T; Missense mutation; Mutation; Mutation rates; p53 Protein; Patients; Point mutation; Signal transduction; T cell receptors; targeted next‐generation sequencing; Tumors
• ISSN: 1099-498X; 1521-2254
• DOI: 10.1002/jgm.3420

Background The present study aimed to explore the genomic profile in a cohort of Chinese patients with breast cancer (BC), as well as provide potential strategies for clinic treatment in specific subset of BC patients. Methods Paired samples from 46 BC patients were subjected to DNA extraction and 537 gene targeted next‐generation sequencing. Results In total, 742 somatic mutations were detected in these patients, which involved 303 genes. TP53 and PIK3CA were the most frequently mutated genes, with a mutation rate of 45.65% and 26.09%. C>T, T>C and C>A comprised the main single nucleotide base variation for this Chinese cohort. Triple negative breast cancer (TNBC) group had more TP53‐mutated patients than the Non‐TNBC group (p = 0.0229). In addition, the cohort was also divided into ‘Young’ and ‘Old’ groups based on the age of onset. Compared with the ‘Young’ group, the ‘Old’ group had more frameshift mutations (p = 0.0190), less missense mutations (p = 0.0269) and more HOXA11‐mutated patients (p = 0.0197). Additionally, the HOXA11mt (HOXA11 gene mutated) group had more frameshift mutations than the HOXA11wt (HOXA11 gene without mutation) group (p