Pretreatment of Jerusalem artichoke stalk using hydroxylammonium ionic liquids and their influences on 2,3-butanediol fermentation by Bacillus subtilis

• Published in: Bioresource technology 2022-06, Vol.354, p.127219-127219, Article 127219
• Main Authors: An, Yu-Meng, Zhuang, Jing, Li, Yan, Dai, Jian-Ying, Xiu, Zhi-Long
• Format: Article
• Language: English
• Subjects: 2,3-Butanediol; Cell toxicity; Hydroxylammonium ionic liquids; Jerusalem artichoke stalk; Lignocellulose pretreatment
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2022.127219

•Pretreatment of Jerusalem artichoke stalk by hydroxylammonium ILs were compared.•Production of acetoin and 2,3-BD increased at 0.1 mol/L ethanolammonium acetate.•87% cellulose and 75% hemicellulose recovered and 57% lignin removed at 170 °C.•Glucose yield of 64% and xylose of 66% obtained in 24-h hydrolysis by cellulase. Pretreatment of lignocellulose is a vital step for biological production of bio-chemicals and bio-fuels. In this work, the pretreatment of Jerusalem artichoke stalk (JAS) by hydroxylammonium ionic liquids was evaluated based on pretreatment efficiency including polysaccharide recovery and enzymatic digestibility, and influence of ionic liquids on 2,3-butanediol fermentation using Bacillus subtilis. The results showed ethanolammonium acetate (EOAA) was efficient in JAS pretreatment, and maximum cell density was increased 25% when EOAA concentration was not greater than 0.3 mol/L in medium, while the total concentration of acetoin and 2,3-butanediol was 15% greater than the control at 0.1 mol/L EOAA. After the pretreatment under optimized conditions of 170 °C for 5-h and liquid–solid ratio of 18, about 87% cellulose and 75% hemicellulose were recovered, and glucose yield of 64% and xylose of 66% were obtained after 24-h hydrolysis of JAS residue by cellulase (15 FPU/g) with solid loading of 10 wt%.