Airy-like beam-based light-sheet microscopy with improved FOV for zebrafish intracerebral hemorrhage

Airy light-sheet microscopy is rapidly gaining importance for imaging intact biological specimens because of the rapid speed, high resolution, and wide field nature of the imaging method. However, the depth of field (DOF) of the detection objective imposes limitations on the modulation transfer func...

Full description

Saved in:
Bibliographic Details
Published in:Optics express 2022-04, Vol.30 (9), p.14709-14722
Main Authors: Liu, Pengfei, Chen, Hongyu, Pang, Meijun, Liu, Xiuyun, Wang, Jiwei, Zhang, Xiao-Dong, Ming, Dong
Format: Article
Language:English
Subjects:
Animals
Cerebral Hemorrhage - diagnostic imaging
Larva
Microscopy - methods
Photons
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Airy light-sheet microscopy is rapidly gaining importance for imaging intact biological specimens because of the rapid speed, high resolution, and wide field nature of the imaging method. However, the depth of field (DOF) of the detection objective imposes limitations on the modulation transfer function (MTF) of the light sheet, which in turn affects the size of the field of view (FOV). Here we present an optimized phase modulation model, based on 'Airy-like' beam family, to stretch the curved lobes, which brings a wider FOV while maintaining high resolution. In addition, we further develop a planar 'Airy-like' light-sheet by two-photon excitation which can avoid the deconvolution process. We validated the new imaging method by performing a real-time monitoring of the dynamic process of cerebral hemorrhage in zebrafish larva. The proposed Airy-like beam-based light-sheet microscopy has great potential to be applied to the precise screening of cerebral hemorrhage-related drugs to help precision medicine in the future.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.451919