Expression of LncRNAs in HPV-induced Carcinogenesis and Cancer Cachexia: A Study in K14-HPV16 Mice

• Published in: Anticancer research 2022-05, Vol.42 (5), p.2443-2460
• Main Authors: Dias, Tânia R, Santos, Joana M O, Estêvão, Diogo, Costa, Natália R, Mestre, Verónica F, Medeiros-Fonseca, Beatriz, Bastos, Margarida M S M, Oliveira, Paula A, Gil DA Costa, Rui M, Medeiros, Rui
• Format: Article
• Language: English
• Subjects: Animal tissues; Animals; Cachexia; Cachexia - genetics; Cancer; Carcinogenesis; Carcinogenesis - genetics; Carcinogens; Chest; Cyclin-dependent kinase inhibitor p21; Ear; Female; Gastrocnemius muscle; Human papillomavirus; Human papillomavirus 16 - genetics; Human papillomavirus 16 - metabolism; Humans; Male; Mice; Muscles; Non-coding RNA; Papillomavirus Infections - complications; Papillomavirus Infections - genetics; Papillomavirus Infections - metabolism; Penis; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Skin; Tongue
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.15723

To evaluate the expression of lincRNA-p21, H19, EMX2OS, SNHG12 and MALAT1 in a mouse model of human papillomavirus 16 (HPV16)-induced carcinogenesis and cachexia. Chest skin, ear, tongue, penis and gastrocnemius muscle samples from wild-type mice (HPV-) and K14-HPV16 male mice (HPV+) were collected to evaluate the expression of the selected lncRNAs using real-time PCR (qPCR). In chest skin and ear, H19, SNHG12, EMX2OS and lincRNA-p21 were down-regulated in HPV+ versus HPV- mice. In tongue and penile tissues, there was only down-regulation of lincRNA-p21 in HPV+ mice. Additionally, in penile tissue, lincRNA-p21 expression decreased in HPV-induced lesions comparing with normal tissues. In gastrocnemius muscle, MALAT1 was up-regulated and lincRNA-p21 was down-regulated in HPV+ versus HPV-mice. H19, SNHG12, EMX2OS and lincRNA-p21 may be involved in HPV-induced carcinogenesis. In addition, MALAT1 and lincRNA-p21 may play a role in muscle wasting and contribute to cancer cachexia.