Sodium Butyrate Induces Hepatic Differentiation of Mesenchymal Stem Cells in 3D Collagen Scaffolds

Stem cell-based therapy is considered an attractive tool to overcome the burden of liver diseases. However, efficient hepatic differentiation is still a big challenge for the research community. In this study, we explored a novel method for differentiation of bone marrow-derived mesenchymal stem cel...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2022-08, Vol.194 (8), p.3721-3732
Main Authors: Rashid, Saman, Salim, Asmat, Qazi, Rida -e- Maria, Malick, Tuba Shakil, Haneef, Kanwal
Format: Article
Language:English
Subjects:
Acetylation
Biochemistry
Biomedical materials
Biotechnology
Bone marrow
Cell culture
Cell differentiation
Cell surface
Chemistry
Chemistry and Materials Science
Collagen
Cytokines
Differentiation
Flow cytometry
Growth factors
Histone deacetylase
Histones
Immunocytochemistry
Liver diseases
Mesenchymal stem cells
Original Article
Sodium
Sodium butyrate
Stem cells
Surface markers
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Summary:Stem cell-based therapy is considered an attractive tool to overcome the burden of liver diseases. However, efficient hepatic differentiation is still a big challenge for the research community. In this study, we explored a novel method for differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into hepatic-like cells using 3D culture conditions and histone deacetylase inhibitor, sodium butyrate (NaBu). MSCs were characterized by the presence of cell surface markers via immunocytochemistry, flow cytometry, and by their potential for osteogenic, adipogenic, and chondrogenic differentiation. MSCs were treated with 1mM NaBu in 2D and 3D environments for 21 days. The hepatic differentiation was confirmed by qPCR and immunostaining. According to qPCR data, the 3D culture of NaBu-treated MSCs has shown significant upregulation of hepatic gene, CK-18 ( P < 0.01), and hepatic proteins, AFP ( P < 0.01) and ALB ( P < 0.01). In addition, immunocytochemistry analysis showed significant increase ( P < 0.05) in the acetylation of histones (H3 and H4) in NaBu-pretreated cells. It can be concluded from the study that NaBu-treated MSCs in 3D culture conditions can induce hepatic differentiation without the use of additional cytokines and growth factors. The method shown in this study represents an improved protocol for hepatic differentiation and could contribute to improvement in future cell-based therapeutics.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-022-03941-5