Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing

• Published in: Parasitology research (1987) 2022-07, Vol.121 (7), p.1993-2008
• Main Authors: Xiao, Jing, Zhu, Yazhou, Wu, Jianwen, Bai, Min, Xin, Yunzhuo, Wang, Qiang, Zhao, Jiaqing
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Body fluids; Cell differentiation; Echinococcosis; Echinococcus granulosus; Exosomes; Forkhead protein; Genetic research; Genomes; Health aspects; Helper cells; Host-parasite interactions; Immune response; Immunology; Immunology and Host-Parasite Interactions - Original Paper; Infection; Infections; Lymphocytes T; MAP kinase; Medical Microbiology; Medical research; Medicine, Experimental; Microbiology; Microenvironments; MicroRNA; MicroRNAs; miRNA; Next-generation sequencing; Parasites; Peripheral blood; Signal transduction; Wnt protein; Zoonoses
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-022-07536-1

Echinococcosis is a worldwide zoonosis. The mechanism of the establishment, growth, and persistence of parasites in the host has not been fully understood. Exosomes are found to be a way of information exchange between parasites and hosts. They exist in various body fluids widely. There are few studies on host-derived exosomes and their miRNA expression profiles at different infection time points. In this study, BALB/c mice were intraperitoneally infected with protricercariae. Exosomes were extracted from plasma (0, 3, 9, and 20 weeks post infection), and the expression profiles of exosome miRNA in the peripheral blood of mice were determined using RNA-sequencing. Compared to the 0 week groups, 24, 35, and 22 differentially expressed miRNAs were detected in infected mouse at the three infection stages, respectively. The results showed that there were significant differences in the miRNAs of exosomes at different infection time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used to annotate the different miRNAs. The results showed that the biological pathways of parasites changed significantly at different stages of infection, with many significant and abundant pathways involved in cell differentiation, inflammation, and immune response, such as MAPK signaling pathway, Th17 cell differentiation, Wnt signaling pathway, FoxO signaling pathway, Notch signaling pathway, etc. These results suggest that miRNA may be an important regulator of interactions between Echinococcus granulosus and host. The data provided here provide valuable information to increase understanding of the regulatory function of microRNAs in the host microenvironment and the mechanism of host-parasite interaction. This may help us to find targets for Echinococcus granulosus to escape host immune attack and control Echinococcus granulosus infection in the future.