Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

• Published in: Journal of the science of food and agriculture 2022-11, Vol.102 (14), p.6263-6272
• Main Authors: Wang, Dingkang, Mi, Ting, Huang, Jun, Zhou, Rongqing, Jin, Yao, Wu, Chongde
• Format: Article
• Language: English
• Subjects: Abiotic stress; Amino acids; Carbohydrates; Cell membranes; cell morphology; Cytology; Fatty acids; Fermented food; Fluidity; Glycerol; Growth rate; intracellular metabolites; Membrane fluidity; Metabolites; Metabolomics; Nucleotides; Organic acids; Salinity tolerance; Salt tolerance; Stearic acid; Surface roughness; Traditional foods; Xanthohumol; Xylitol; Zygosaccharomyces rouxii
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.11975

BACKGROUND Zygosaccharomyces rouxii plays an irreplaceable role in the manufacture of traditional fermented foods, which are produced in a high‐salt environment. However, there is little research on strategies for improving salt tolerance of Z. rouxii. RESULTS In this study, metabolomics was used to reveal the changes in intracellular metabolites under salt stress, and the results show that most of the carbohydrate contents decreased, the contents of xanthohumol and glycerol increased (fold change 4.07 and 5.35, respectively), while the contents of galactinol, xylitol and d‐threitol decreased (fold change −9.43, −5.83 and −3.59, respectively). In addition, the content of four amino acids and six organic acids decreased, while that of the ten nucleotides increased. Notably, except for stearic acid (C18:0), all fatty acid contents increased. Guided by the metabolomics results, the effect of addition of seven exogenous fatty acids (C12:0, C14:0, C16:0, C18:0, C16:1, C18:1, and C18:2) on the salt tolerance of Z. rouxii was analyzed, and the results suggested that four exogenous fatty acids (C12:0, C16:0, C16:1, and C18:1) can increase the biomass yield and maximum growth rate. Physiological analyses demonstrated that exogenous fatty acids could regulate the distribution of fatty acids in the cell membrane, increase the degree of unsaturation, improve membrane fluidity, and maintain cell integrity, morphology and surface roughness. CONCLUSION These results are applicable to revealing the metabolic mechanisms of Z. rouxii under salt stress and screening potential protective agents to improve stress resistance by adding exogenous fatty acids. © 2022 Society of Chemical Industry.