Accounting for co-extractable compounds (blank correction) in spectrophotometric measurement of extractable and total-bound proanthocyanidin in Leucaena spp

Methods to account for the spectral interference of co-extractable compounds (blank correction) in the spectrophotometric analysis of both extractable and bound proanthocyanidin (PA) using the proanthocyanidin (butanol/HCl) assay were evaluated. Crude extractable and bound PA sample matrices of PA-f...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2002-06, Vol.82 (8), p.860-868
Main Authors: Dalzell, Scott A, Kerven, Graham L
Format: Article
Language:English
Subjects:
absorbance
anthocyanidins
Biological and medical sciences
blank correction
bound
butanol
co-extractable compounds
computer software
condensed tannin
extractable
fibre-bound
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
hydrochloric acid
insoluble
Leucaena
Leucaena magnifica
methodology
Methods of analysis, processing and quality control, regulation, standards
proanthocyanidin
protein-bound
temperature
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Summary:Methods to account for the spectral interference of co-extractable compounds (blank correction) in the spectrophotometric analysis of both extractable and bound proanthocyanidin (PA) using the proanthocyanidin (butanol/HCl) assay were evaluated. Crude extractable and bound PA sample matrices of PA-free Leucaena magnifica were used. Extractable PA blanks generated in heated 95% butanol/5% H2O reagent underestimated the optical density (absorbance) of co-extractable compounds by 24% (P 
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.1106