Microbial production of 2-pyrone-4,6-dicarboxylic acid from lignin derivatives in an engineered Pseudomonas putida and its application for the synthesis of bio-based polyester

[Display omitted] •Lignin-derived monomers were successfully converted to PDC in Pseudomonas putida.•A metabolically engineered host strain of P. putida increased PDC production.•High titer and yield of PDC were obtained in optimized fed-batch fermentation.•A novel polyester was synthesized using pu...

Full description

Saved in:
Bibliographic Details
Published in:Bioresource technology 2022-05, Vol.352, p.127106-127106, Article 127106
Main Authors: Lee, Siseon, Jung, Ye Jean, Park, Si Jae, Ryu, Mi-Hee, Kim, Joo Eon, Song, Hye Min, Kang, Kyoung Hee, Song, Bong Keun, Sung, Bong Hyun, Kim, Yong Hwan, Kim, Hee Taek, Joo, Jeong Chan
Format: Article
Language:English
Subjects:
2-pyrone-4
6-dicarboxylic acid
batch fermentation
Bio-based polyester
biochemical pathways
Biological funneling
bioplastics
Dicarboxylic Acids - metabolism
ferulic acid
genes
Lignin
Lignin - metabolism
p-coumaric acid
polyesters
Polyesters - metabolism
protocatechuic acid
Pseudomonas putida
Pseudomonas putida - genetics
Pseudomonas putida - metabolism
Pyrones
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Lignin-derived monomers were successfully converted to PDC in Pseudomonas putida.•A metabolically engineered host strain of P. putida increased PDC production.•High titer and yield of PDC were obtained in optimized fed-batch fermentation.•A novel polyester was synthesized using purified PDC, exhibiting thermal stability. Lignin valorization depends on microbial upcycling of various aromatic compounds in the form of a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric compounds via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic acid (PDC), which has been considered a promising building block for bioplastics. The biosynthetic pathway for PDC production was established by introducing the heterologous ligABC genes under the promoter Ptac in a strain lacking pcaGH genes to accumulate a precursor of PDC, i.e., protocatechuic acid. Based on the culture optimization, fed-batch fermentation of the final strain resulted in 22.7 g/L PDC with a molar yield of 1.0 mol/mol and productivity of 0.21 g/L/h. Subsequent purification of PDC at high purity was successfully implemented, which was consequently applied for the novel polyester.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2022.127106