Morphological, molecular, and biochemical study of cyanobacteria from a eutrophic Algerian reservoir (Cheffia)

• Published in: Environmental science and pollution research international 2022-04, Vol.29 (19), p.27624-27635
• Main Authors: Benredjem, Lamia, Berredjem, Hajira, Abdi, Akila, Casero, Maria Cristina, Quesada, Antonio, Fosso, Bruno, Marzano, Marinella, Pesole, Graziano, Azevedo, Joana, Vasconcelos, Vitor
• Format: Article
• Language: English
• Subjects: Algeria; Aphanizomenon; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Community composition; Community structure; Cyanobacteria; Cyanobacteria - chemistry; DNA barcoding; Earth and Environmental Science; Ecosystem; ecosystems; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental Monitoring - methods; Environmental science; Eutrophic environments; Eutrophication; Gene sequencing; Genera; Genotypes; genus; High-performance liquid chromatography; irrigation; Irrigation water; Liquid chromatography; Microcystin-LR; Microcystins; Microcystins - analysis; Microcystis; Microcystis - genetics; Morphology; Next-generation sequencing; nutrient content; Planktothrix; Research Article; Reservoirs; RNA, Ribosomal, 16S - genetics; rRNA 16S; species; surface water; Tandem Mass Spectrometry; Taxonomy; temperature; Toxins; Waste Water Technology; Water - analysis; Water bodies; Water Management; Water Pollution Control; Water sampling; Water supply
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-17528-w

The cyanobacteria management in water bodies requires a deep knowledge of the community composition. Considering the reliable and thorough information provided by the polyphasic approach in cyanobacteria taxonomy, here we assess the cyanobacterial community structure of the Cheffia reservoir from Algeria. Cyanobacteria were identified on the basis of morphological traits and next-generation sequencing (NGS); toxins-related genes were localized in addition to the identification of toxins; temperature and nutrient level of water samples were also determined. The polyphasic approach was essential for cyanobacteria investigation; 28 genera were identified through 16S rRNA metabarcoding with the dominance of taxa from Microcystis (34.2%), Aphanizomenon (20.1%), and Planktothrix (20.0%), and morphological analysis revealed the association in this water body of five species within the genus Microcystis : M. aeruginosa , M. novacekii , M. panniformis , M. ichthyoblabe , and M. flos-aquae . The presence of mcyE genotypes was detected; moreover, HPLC–PDA and LC–ESI–MS/MS revealed the production of microcystin-LR. Results obtained in our study are very important since this ecosystem is used for water supply and irrigation; as a consequence, a good water management plan is essential.