Skeletal muscle gene expression in older adults with type 2 diabetes mellitus undergoing calorie-restricted diet and recreational sports training - a randomized clinical trial

• Published in: Experimental gerontology 2022-07, Vol.164, p.111831-111831, Article 111831
• Main Authors: da Silva Soares, Diana Bento, Shinjo, Samuel Katsuyuki, Santos, Aritânia Sousa, de Cassia Rosa de Jesus, Joyce, Schenk, Simon, de Castro, Gabriela Salim, Zanoteli, Edmar, Krustrup, Peter, da Silva, Maria Elizabeth Rossi, de Sousa, Maysa Vieira
• Format: Article
• Language: English
• Subjects: Exercise; Interleukin-15; Muscle proteins; Nutritional intervention; Overweight; Ubiquitin-protein ligases
• ISSN: 0531-5565; 1873-6815
• DOI: 10.1016/j.exger.2022.111831

This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients. Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m2) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables. Total body weight was reduced in ~4 kg representing body fat mass in all groups after 12-weeks (P