Diabetes negatively affects tooth enamel and dentine microhardness: An in-vivo study

• Published in: Archives of oral biology 2022-07, Vol.139, p.105434-105434, Article 105434
• Main Authors: Saghiri, Mohammad Ali, Sheibani, Nader, Kawai, Toshihisa, Nath, Devyani, Dadvand, Sahar, Amini, Saeid B., Vakhnovetsky, Julia, Morgano, Steven M.
• Format: Article
• Language: English
• Subjects: Dentine microhardness; Diabetes mellitus; Enamel; Streptozotocin
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2022.105434

This study was performed to evaluate the effect of type 1 diabetes mellitus (T1DM) on the microhardness of tooth enamel and dentine in mice. Seventy male C57BL/6 J mice were used in this study. Thirty-five mice were rendered diabetic by administration of streptozotocin (STZ), and the remaining animals received citrate buffer (normal/non-diabetic). In each group, specimens were divided into 7 subgroups of 5 mice based on the time points 0, 1, 4, 8, 12, 20, and 28 weeks. The microhardness value (MHV) of the second molars’ enamel and root dentine were tested with a Vickers microhardness tester. Five specimens from each subgroup were evaluated for dentinal tubular density by scanning electron microscope (SEM) and color dot map analysis to determine the color intensity of strontium (Sr) and magnesium (Mg) by using ImageJ software. The MHV of enamel was significantly reduced in STZ specimens in time points of 12 weeks (STZ: 274.39 ± 15.42, normal: 291.22 ± 15.28), 20 weeks (STZ: 247.28 ± 19.65, normal: 290.68 ± 11.52), and 28 weeks (STZ: 232.87 ± 15.07, normal: 282.76 ± 10.36) (P