Two typical acyl-CoA-binding proteins (ACBPs) are required for the asexual development and virulence of Phytophthora sojae

•PsACBP2 but not PsACBP1 is important for growth in Phytophthora sojae.•Both PsACBP1 and PsACBP2 contribute to normal zoospore development.•PsACBP1 and PsACBP2 are both required for pathogenicity of Phytophthora sojae.•PsACBP2 plays an important role in biogenesis of lipid droplets of Phytophthora s...

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Published in:Fungal genetics and biology 2022-07, Vol.161, p.103695-103695, Article 103695
Main Authors: Pei, Yong, Si, Jierui, Navet, Natasha, Ji, Peiyun, Zhang, Xiong, Qiao, Huijun, Xu, Ruofei, Zhai, Ying, Miao, Jianqiang, Tyler, Brett M., Dou, Daolong
Format: Article
Language:English
Subjects:
Acyl-CoA-binding protein (ACBP)
Animals
Coenzyme A - metabolism
Diazepam Binding Inhibitor - genetics
Diazepam Binding Inhibitor - metabolism
Glycine max - genetics
Lipid bodies
Mammals - metabolism
Phytophthora
Phytophthora sojae
Virulence
Virulence - genetics
Zoospore production
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Summary:•PsACBP2 but not PsACBP1 is important for growth in Phytophthora sojae.•Both PsACBP1 and PsACBP2 contribute to normal zoospore development.•PsACBP1 and PsACBP2 are both required for pathogenicity of Phytophthora sojae.•PsACBP2 plays an important role in biogenesis of lipid droplets of Phytophthora sojae. Being found in all eukaryotes investigated, acyl-CoA-binding proteins (ACBPs) participate in lipid metabolism via specifically binding acyl-CoA esters with high affinity. The structures and functions of ACBP family proteins have been extensively described in yeasts, fungi, plants and mammals, but not oomycetes. In the present study, seven ACBP genes named PsACBP1-7 were identified from the genome of Phytophthora sojae, an oomycete pathogen of soybean. CRISPR-Cas9 knockout mutants targeting PsACBP1 and PsACBP2 were created for phenotypic assays. PsACBP1 knockout led to defects in sporangia production and virulence. PsACBP2 knockout mutants exhibited impaired vegetative growth, zoospore production, cyst germination and virulence. Moreover, Nile red staining of PsACBP2 knockout and over-expression lines showed that PsACBP2 is involved in the formation of lipid bodies in P. sojae. Our results demonstrate that two ACBP genes are differently required for growth and development, and both are essential for virulence in P. sojae.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2022.103695