Antioxidant capacity of Myrciaria cauliflora seed extracts by spectrophotometric, biochemical, and electrochemical methods and its protective effect against oxidative damage in erythrocytes

• Published in: Journal of food biochemistry 2022-09, Vol.46 (9), p.e14222-n/a
• Main Authors: Hacke, Ana Carolina Mendes, Valério, Taynara Pacheco, Cubo, Mateus Flórido, Lima, Dhésmon, Pessôa, Christiana Andrade, Vellosa, José Carlos Rebuglio, Pereira, Romaiana Picada
• Format: Article
• Language: English
• Subjects: differential pulse voltammetry; fruit by‐product; lipid peroxidation; polyphenols; radical scavenger
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/jfbc.14222

This study aimed to investigate the antioxidant activity of extracts obtained from jabuticaba (Myrciaria cauliflora) seeds. Ethanolic (ETJS), methanolic (MEJS), aqueous (AQJS), and propanone (PRJS) extracts was assessed by measuring spectrophotometrically their ability to scavenge DPPH·, ABTS·+, HOCl, and O2·− radicals. Electrochemical methods were employed, and the obtained data presented a good correlation with the radical scavenging results. The extracts were also able to attenuate lipid peroxidation induced by Fe2+ ions in phospholipids due to their chelation ability. The extracts protected human erythrocytes against oxidative cellular damage caused by AAPH, which was confirmed by using FESEM analysis. PRJS extract demonstrated the highest effect in all assays used in this work. Our findings prove that jabuticaba seeds are an important source of antioxidants which act by different mechanisms. This study opens new frontiers regarding the use of this fruit byproduct as a food additive. Practical applications Jabuticaba seeds are usually discarded as waste by food industries, but they are rich in bioactive products and present interesting biological properties. Herein, we demonstrated that their extracts show remarkable antioxidant power against different reactive oxygen species, which are involved in several human pathologies. In this way, this by‐product can be further used in the development of products to protect the human body against diseases related to oxidative stress. Electrochemical assays confirmed the antioxidant power of jabuticaba seed extracts. Extracts prevented lipid peroxidation induced by Fe2+ in phospholipids. Jabuticaba seed extracts prevented AAPH‐induced oxidative damages in erythrocytes.