Discontinuously Dewetting Solvent Arrays: Droplet Formation and Poly-Synchronous Surface Extraction for Mass Spectrometry Imaging Applications

We describe a new liquid tissue stamping method called poly-synchronous surface extraction (PSSE) that utilizes an omniphobic substrate patterned with hydrophilic surface energy traps (SETs), which when wet with a solvent form a dense microdroplet array. When contacted with a tissue sample, each dro...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-05, Vol.94 (20), p.7219-7228
Main Authors: McEwen, Rory A. H., Hermann, Matthias, Metwally, Haidy, Donovan, Katherine, Liu, Chang, Le Blanc, J. C. Yves, Covey, Thomas R., Oleschuk, Richard
Format: Article
Language:English
Subjects:
Analytical chemistry
Arrays
Chemical compounds
Chemistry
Droplets
Fragaria ananassa
Imaging techniques
Ionization
Ions
Mass spectrometry
Mass spectroscopy
Optimization
Sample preparation
Scientific imaging
Solvents
Spectroscopy
Substrates
Surface energy
Surface properties
Tissues
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Summary:We describe a new liquid tissue stamping method called poly-synchronous surface extraction (PSSE) that utilizes an omniphobic substrate patterned with hydrophilic surface energy traps (SETs), which when wet with a solvent form a dense microdroplet array. When contacted with a tissue sample, each droplet locally extracts analytes from the tissue surface, which subsequentially can be analyzed by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-IMS) or ambient ionization-MS techniques. Optimization of the patterned surface with six different solvents was carried out to increase the droplet density, height, and reproducibility of volume deposition. Once optimized, sister slices of a strawberry (Fragaria × ananassa) were spatially extracted using the PSSE technique and the chemical distribution of selected compounds was analyzed with both MALDI-IMS and a lower resolution but faster ambient liquid microjunction surface sampling probe (LMJ-SSP) approach. Heat maps for target analytes for the PSSE approach are compared to those produced using traditional MALDI-IMS analysis. The PSSE method aligned well with direct analysis and demonstrated the potential to increase the speed of ambient MS tissue imaging techniques by decreasing the number of steps required for sample preparation.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c00161