Finding the Light Emission Stimulator of Neonothopanus nambi Basidiomycete and Studying Its Properties

• Published in: Doklady. Biochemistry and biophysics 2022-04, Vol.503 (1), p.80-84
• Main Authors: Ronzhin, N. O., Posokhina, E. D., Mogilnaya, O. A., Bondar, V. S.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Heat treatments; Life Sciences; Luminescence; Molecular Biology; Neonothopanus nambi; β-Glucosidase
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672922020120

A stimulator of light emission of the fungus was found in an aqueous extract from mycelium of the luminous basidiomycete Neonothopanus nambi after its treatment with β-glucosidase. The addition of the extract to the luminous mycelium increases the level of light emission from several times to 1.5 orders of magnitude or more. The luminescence stimulator is a low-molecular-weight thermostable compound: it is detected in the permeate after filtering the extract through a 10-kDa cutoff membrane and it retains the stimulating effect after heat treatment at 100°C for 5 min. In the absorption spectrum of the aqueous sample of the stimulator, two main peaks are observed in the shortwave region (205 and 260 nm) and a shoulder in the range of 350–370 nm can be seen. The luminescence stimulator exhibits blue fluorescence with an emission maximum at 440 nm when excited at 360 nm. It was established that the luminescence-stimulating component is not a substrate (or its precursor) of the luminescent system of the N. nambi fungus.