Molecular cloning, characterization and expression analysis of CXCR3a and CXCR3b from Nile tilapia (Oreochromis niloticus)

The CXC chemokine receptors (CXCRs) are members of the seven transmembrane (7‐TM) G‐protein‐coupled receptor superfamily that involves innate and adaptive immune systems. In this study, CXCR3a and CXCR3b from Nile tilapia (Oreochromis niloticus) were cloned and identified, designated as OnCXCR3a and...

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Bibliographic Details
Published in:Journal of fish biology 2022-09, Vol.101 (3), p.431-440
Main Authors: Gao, Along, Wu, Liting, Li, Lan, Han, Biao, Ye, Jianmin
Format: Article
Language:English
Subjects:
Adaptive systems
Amino acids
Analysis
chemokine receptor
Chemokine receptors
Chemokines
Cloning
CXC chemokine receptors
CXCR3
Freshwater fishes
Immune response
immune responses
Immune system
Leukocytes
Lipopolysaccharides
Marine fishes
mRNA
Nucleotide sequence
Open reading frames
Oreochromis niloticus
Ponds
Proteins
Tilapia
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Summary:The CXC chemokine receptors (CXCRs) are members of the seven transmembrane (7‐TM) G‐protein‐coupled receptor superfamily that involves innate and adaptive immune systems. In this study, CXCR3a and CXCR3b from Nile tilapia (Oreochromis niloticus) were cloned and identified, designated as OnCXCR3a and OnCXCR3b. The open reading frames of OnCXCR3a and OnCXCR3b were 1074 and 1080 bp, encoding the predicted proteins of 357 and 359 amino acids, respectively. Multiple alignment analysis of OnCXCR3a‐ and OnCXCR3b‐deduced protein sequences with the mammalian and bird sequences indicated the presence of typical structural features of chemokine receptors, including a 7‐TM domain and conserved motifs. Quantitative real‐time PCR analysis revealed that OnCXCR3a and OnCXCR3b were constitutively expressed in a wide range of tissues. When stimulated with Streptococcus agalactiae, Aeromonas hydrophila, polyinosinic:polycytidylic acid and lipopolysaccharide in vivo or in vitro on leukocytes, the mRNA levels of OnCXCR3a and OnCXCR3b were significantly upregulated. Overall, these results indicated that OnCXCR3s might be involved in host immune responses in Nile tilapia.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.15083