Defining the link between oxidative stress, behavioural reproductive suppression and heterothermy in the Natal mole-rat (Cryptomys hottentotus natalensis)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2022-08, Vol.261, p.110753-110753, Article 110753
• Main Authors: Jacobs, Paul J., Finn, Kyle T., van Vuuren, Andries Koch Janse, Suess, Tobias, Hart, Daniel William, Bennett, Nigel Charles
• Format: Article
• Language: English
• Subjects: Exercise-induced oxidative damage; Heterothermy; Hormesis; Mole-rat; Oxidative stress; Reproductive suppression
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2022.110753

Sub-lethal effects, such as oxidative stress, can be linked to various breeding and thermophysiological strategies, which themselves can be linked to seasonal variability in abiotic factors. In this study, we investigated the subterranean, social living Natal mole-rat (Cryptomys hottentotus natalensis), which, unlike other social mole-rat species, implements heterothermy seasonally in an attempt to avoid exercise-induced hyperthermia and relies solely on behavioural reproductive suppression to maintain reproductive skew in colonies. Subsequently, we investigated how oxidative stress varied between season, sex and breeding status in Natal mole-rats. Oxidative markers included total oxidant status (TOS measure of total peroxides present), total antioxidant capacity (TAC), OSI (oxidative stress index) and malondialdehyde (MDA) to measure oxidative stress. Breeding and non-breeding mole-rats of both sexes were captured during the summer (wet season) and winter (dry season). Seasonal environmental variables (air temperature, soil temperature and soil moisture) had a significant effect on TOS, OSI and MDA, where season affected each sex differently. Unlike other social mole-rat species that use both physiological and behavioural means of reproductive suppression, no oxidative costs to reproduction were present in the Natal mole-rats. Males had significantly higher MDA than females, which was most apparent in summer (wet season). We conclude that the significant oxidative damage in males is a consequence of exercise-induced oxidative stress, exacerbated by increased burrow humidities and poorer heat dissipation abilities as a function of body mass. This study highlights the importance of both breeding and thermophysiological strategies in affecting oxidative stress. [Display omitted] •Seasonal factors significantly affected oxidative stress.•Male body size is positively correlated to oxidative damage.•Significant oxidative damage in males linked to exercised-induced oxidative stress.•Female reproductive strategies mediated by seasonal factors and reproductive state.•First mole-rat to demonstrate sex differences in exercise-induced oxidative stress.