Reduced pH is the primary factor promoting humic acid formation during hyperthermophilic pretreatment composting

• Published in: Journal of environmental management 2022-08, Vol.316, p.115215-115215, Article 115215
• Main Authors: Cao, Yun, Gu, Junyu, Zhang, Jing, Chen, Bao, Xu, Yueding, Liu, Dongyang, Hu, Hangwei, Huang, Hongying
• Format: Article
• Language: English
• Subjects: Bacterial community; Co-60 radiation; Fluorescence excitation emission matrix (EEM); Humification; Hyperthermophilic pretreatment composting
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2022.115215

Hyperthermophilic pretreatment composting (HPC) has the advantages of enhanced composting efficiency and accelerated humic substance (HS) over conventional composting (CC). However, the mechanisms towards the accelerated humification process by HPC are still not clear. By means of sterilization technology, the roles of abiotic and biotic components on the formation of HS can be distinguished. The study investigated the humification degree and the succession of microbial community during HPC of pig manure. The mechanisms underlying the accelerated humification by HPC was identified using gamma sterilization. Results showed that HS content increased significantly by 13.72% in HPC and 29.93% in sterilized HPC inoculated with 1% CC (HPC_I), compared with 8.76% increase in CC and 7.12% increase in sterilized CC inoculated with 1% HPC during composting (CC_I). Compared with CC and CC_I, stronger intensities of HA-like and fulvic acid-like components were observed in HPC and HPC_I. Results showed that physicochemical properties, especially pH, were the key factors in accelerating the humification in HPC, while both physicochemical properties and microbial community contributed to the HA formation in CC. The study contributed to a better understanding of the mechanism towards the accelerated humification degree in HPC. [Display omitted] •Hyperthermophilic pretreatment (HP) enhances formation of humic acid by 76.68%.•A mechanism for enhanced humification is proposed by employing gamma sterilization.•Spore-forming thermophilic bacteria is positively related to the humification.•Reduced pH− primarily contributes to pig manure humification in HP composting.