Extraction of pectic polysaccharides from sugar-beet cell walls

Previous methods of extracting pectin from sugar‐beet have used pulp as the starting material. As the temperature and pressure of the pulping process may modify the architecture of the cell wall, we have adapted a relatively non‐disruptive method to characterise cell wall material (CWM) isolated dir...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2000-01, Vol.80 (1), p.17-28
Main Authors: Marry, Mazz, McCann, Maureen C, Kolpak, Frank, White, Alan R, Stacey, Nicola J, Roberts, Keith
Format: Article
Language:English
Subjects:
acetyl esters
Applied sciences
Biological and medical sciences
cell walls
Exact sciences and technology
ferulic acid
feruloyl esters
Food industries
Fourier transform infrared (FTIR) microspectroscopy
Fundamental and applied biological sciences. Psychology
GC-MS
methyl esters
pectin
Physicochemistry of polymers
Polysaccharides
sugar linkage analysis
sugar-beet
Synthetic biopolymers
Use and upgrading of agricultural and food by-products. Biotechnology
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Summary:Previous methods of extracting pectin from sugar‐beet have used pulp as the starting material. As the temperature and pressure of the pulping process may modify the architecture of the cell wall, we have adapted a relatively non‐disruptive method to characterise cell wall material (CWM) isolated directly from the sugar‐beet. Cell walls from mature sugar‐beets (Beta vulgaris L Aztec) were sequentially extracted four times with imidazole and twice with sodium carbonate to produce six heterogeneous pectic polysaccharide extracts, and with KOH to produce a hemicellulosic extract which was predominantly xylans. Heterogeneity of the extracted pectins was indicated by differences in FTIR spectra, uronic acid content, % methyl esterification, % feruloylation, % acetylation, molecular weight distribution and neutral sugar composition. The highest proportion of feruloyl esters was found in polysaccharides solubilised by the second sodium carbonate extraction. Anion exchange chromatography of these polysaccharides gave three fractions, one of which contained most of the feruloyl ester. These results indicate that feruloyl esters are not randomly distributed among the different pectic polysaccharides in the sugar‐beet cell wall, and that esterification is likely to be dependent on the local sugar sequence or conformation. © 2000 Society of Chemical Industry
ISSN:0022-5142
1097-0010
DOI:10.1002/(SICI)1097-0010(20000101)80:1<17::AID-JSFA491>3.0.CO;2-4