Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China

Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleot...

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Bibliographic Details
Published in:Archives of virology 2022-07, Vol.167 (7), p.1583-1587
Main Authors: Hua, Mengying, Jiang, Shanshan, Yuan, Enping, Wan, Qionglian, Wang, Liyan, Lu, Yuwen, Zheng, Hongying, Chen, Hairu, Chen, Jianping, Yan, Fei, Wang, Shaoxiang, Peng, Jiejun
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Capsicum annuum
Capsicum frutescens
Cloning
Flowers & plants
Genomes
Infections
Infectious Diseases
Laboratories
Medical Microbiology
Nicotiana benthamiana
Nucleotide sequence
p1 Protein
Pathogenicity
Plant virus diseases
Plant viruses
Potyvirus
Proteins
Virology
Viruses
Yellowing
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Summary:Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L .
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-022-05457-z