Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China

Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleot...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2022-07, Vol.167 (7), p.1583-1587
Main Authors: Hua, Mengying, Jiang, Shanshan, Yuan, Enping, Wan, Qionglian, Wang, Liyan, Lu, Yuwen, Zheng, Hongying, Chen, Hairu, Chen, Jianping, Yan, Fei, Wang, Shaoxiang, Peng, Jiejun
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Capsicum annuum
Capsicum frutescens
Cloning
Flowers & plants
Genomes
Infections
Infectious Diseases
Laboratories
Medical Microbiology
Nicotiana benthamiana
Nucleotide sequence
p1 Protein
Pathogenicity
Plant virus diseases
Plant viruses
Potyvirus
Proteins
Virology
Viruses
Yellowing
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c370t-e35a6bb84e002462d5fdd15c6c27161de6cd9871941786926781f73cc5de648f3
container_end_page 1587
container_issue 7
container_start_page 1583
container_title Archives of virology
container_volume 167
creator Hua, Mengying
Jiang, Shanshan
Yuan, Enping
Wan, Qionglian
Wang, Liyan
Lu, Yuwen
Zheng, Hongying
Chen, Hairu
Chen, Jianping
Yan, Fei
Wang, Shaoxiang
Peng, Jiejun
description Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L .
doi_str_mv 10.1007/s00705-022-05457-z
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2664791053</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2664791053</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-e35a6bb84e002462d5fdd15c6c27161de6cd9871941786926781f73cc5de648f3</originalsourceid><addsrcrecordid>eNp9kU1PXCEUhknTRkfbP9BFQ-KmC1G-4S7NWD8SU7toF12RO1yYwTAwhXtN6rZ_vIyjNXHRDYRznvO-wAvAR4JPCMbqtLYFC4QpRVhwodDDGzAjnFGkVaffghlmmCMtsd4HB7XeYdwKTOyBfSaEVLKTM_BnnlMdy2THkBPMHvYJhuTd9jxV6KcYUXRpOa5aZ4Du8uIbGvvl0g3Qnn89gzbm5B7noF2FGAMsIS3rJo_wPpSmEGqO_eigL3kNf04pNYNNyfchWXcM56uQ-vfgne9jdR-e9kPw4-LL9_kVurm9vJ6f3SDLFB6RY6KXi4XmDmPKJR2EHwYirLRUEUkGJ-3QaUU6TpSWHZVKE6-YtaK1uPbsEHze6Tb_X5Oro1mHal2MfXLtsYZKyVVHsGANPXqF3uWppHa7RilKFKdMN4ruKFtyrcV5sylh3ZffhmCzjcjsIjItIvMYkXloQ5-epKfF2g3_Rp4zaQDbAXWz_UtXXrz_I_sXo3OcmA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2672174238</pqid></control><display><type>article</type><title>Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China</title><source>Springer Link</source><creator>Hua, Mengying ; Jiang, Shanshan ; Yuan, Enping ; Wan, Qionglian ; Wang, Liyan ; Lu, Yuwen ; Zheng, Hongying ; Chen, Hairu ; Chen, Jianping ; Yan, Fei ; Wang, Shaoxiang ; Peng, Jiejun</creator><creatorcontrib>Hua, Mengying ; Jiang, Shanshan ; Yuan, Enping ; Wan, Qionglian ; Wang, Liyan ; Lu, Yuwen ; Zheng, Hongying ; Chen, Hairu ; Chen, Jianping ; Yan, Fei ; Wang, Shaoxiang ; Peng, Jiejun</creatorcontrib><description>Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L .</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-022-05457-z</identifier><identifier>PMID: 35567696</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Brief Report ; Capsicum annuum ; Capsicum frutescens ; Cloning ; Flowers &amp; plants ; Genomes ; Infections ; Infectious Diseases ; Laboratories ; Medical Microbiology ; Nicotiana benthamiana ; Nucleotide sequence ; p1 Protein ; Pathogenicity ; Plant virus diseases ; Plant viruses ; Potyvirus ; Proteins ; Virology ; Viruses ; Yellowing</subject><ispartof>Archives of virology, 2022-07, Vol.167 (7), p.1583-1587</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c370t-e35a6bb84e002462d5fdd15c6c27161de6cd9871941786926781f73cc5de648f3</cites><orcidid>0000-0003-2594-9776</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35567696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hua, Mengying</creatorcontrib><creatorcontrib>Jiang, Shanshan</creatorcontrib><creatorcontrib>Yuan, Enping</creatorcontrib><creatorcontrib>Wan, Qionglian</creatorcontrib><creatorcontrib>Wang, Liyan</creatorcontrib><creatorcontrib>Lu, Yuwen</creatorcontrib><creatorcontrib>Zheng, Hongying</creatorcontrib><creatorcontrib>Chen, Hairu</creatorcontrib><creatorcontrib>Chen, Jianping</creatorcontrib><creatorcontrib>Yan, Fei</creatorcontrib><creatorcontrib>Wang, Shaoxiang</creatorcontrib><creatorcontrib>Peng, Jiejun</creatorcontrib><title>Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L .</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brief Report</subject><subject>Capsicum annuum</subject><subject>Capsicum frutescens</subject><subject>Cloning</subject><subject>Flowers &amp; plants</subject><subject>Genomes</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Laboratories</subject><subject>Medical Microbiology</subject><subject>Nicotiana benthamiana</subject><subject>Nucleotide sequence</subject><subject>p1 Protein</subject><subject>Pathogenicity</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>Potyvirus</subject><subject>Proteins</subject><subject>Virology</subject><subject>Viruses</subject><subject>Yellowing</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kU1PXCEUhknTRkfbP9BFQ-KmC1G-4S7NWD8SU7toF12RO1yYwTAwhXtN6rZ_vIyjNXHRDYRznvO-wAvAR4JPCMbqtLYFC4QpRVhwodDDGzAjnFGkVaffghlmmCMtsd4HB7XeYdwKTOyBfSaEVLKTM_BnnlMdy2THkBPMHvYJhuTd9jxV6KcYUXRpOa5aZ4Du8uIbGvvl0g3Qnn89gzbm5B7noF2FGAMsIS3rJo_wPpSmEGqO_eigL3kNf04pNYNNyfchWXcM56uQ-vfgne9jdR-e9kPw4-LL9_kVurm9vJ6f3SDLFB6RY6KXi4XmDmPKJR2EHwYirLRUEUkGJ-3QaUU6TpSWHZVKE6-YtaK1uPbsEHze6Tb_X5Oro1mHal2MfXLtsYZKyVVHsGANPXqF3uWppHa7RilKFKdMN4ruKFtyrcV5sylh3ZffhmCzjcjsIjItIvMYkXloQ5-epKfF2g3_Rp4zaQDbAXWz_UtXXrz_I_sXo3OcmA</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Hua, Mengying</creator><creator>Jiang, Shanshan</creator><creator>Yuan, Enping</creator><creator>Wan, Qionglian</creator><creator>Wang, Liyan</creator><creator>Lu, Yuwen</creator><creator>Zheng, Hongying</creator><creator>Chen, Hairu</creator><creator>Chen, Jianping</creator><creator>Yan, Fei</creator><creator>Wang, Shaoxiang</creator><creator>Peng, Jiejun</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2594-9776</orcidid></search><sort><creationdate>20220701</creationdate><title>Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China</title><author>Hua, Mengying ; Jiang, Shanshan ; Yuan, Enping ; Wan, Qionglian ; Wang, Liyan ; Lu, Yuwen ; Zheng, Hongying ; Chen, Hairu ; Chen, Jianping ; Yan, Fei ; Wang, Shaoxiang ; Peng, Jiejun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-e35a6bb84e002462d5fdd15c6c27161de6cd9871941786926781f73cc5de648f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brief Report</topic><topic>Capsicum annuum</topic><topic>Capsicum frutescens</topic><topic>Cloning</topic><topic>Flowers &amp; plants</topic><topic>Genomes</topic><topic>Infections</topic><topic>Infectious Diseases</topic><topic>Laboratories</topic><topic>Medical Microbiology</topic><topic>Nicotiana benthamiana</topic><topic>Nucleotide sequence</topic><topic>p1 Protein</topic><topic>Pathogenicity</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>Potyvirus</topic><topic>Proteins</topic><topic>Virology</topic><topic>Viruses</topic><topic>Yellowing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hua, Mengying</creatorcontrib><creatorcontrib>Jiang, Shanshan</creatorcontrib><creatorcontrib>Yuan, Enping</creatorcontrib><creatorcontrib>Wan, Qionglian</creatorcontrib><creatorcontrib>Wang, Liyan</creatorcontrib><creatorcontrib>Lu, Yuwen</creatorcontrib><creatorcontrib>Zheng, Hongying</creatorcontrib><creatorcontrib>Chen, Hairu</creatorcontrib><creatorcontrib>Chen, Jianping</creatorcontrib><creatorcontrib>Yan, Fei</creatorcontrib><creatorcontrib>Wang, Shaoxiang</creatorcontrib><creatorcontrib>Peng, Jiejun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hua, Mengying</au><au>Jiang, Shanshan</au><au>Yuan, Enping</au><au>Wan, Qionglian</au><au>Wang, Liyan</au><au>Lu, Yuwen</au><au>Zheng, Hongying</au><au>Chen, Hairu</au><au>Chen, Jianping</au><au>Yan, Fei</au><au>Wang, Shaoxiang</au><au>Peng, Jiejun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>167</volume><issue>7</issue><spage>1583</spage><epage>1587</epage><pages>1583-1587</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>Chilli ringspot virus (ChiRSV; genus Potyvirus ) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L .</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>35567696</pmid><doi>10.1007/s00705-022-05457-z</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0003-2594-9776</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0304-8608
ispartof Archives of virology, 2022-07, Vol.167 (7), p.1583-1587
issn 0304-8608
1432-8798
language eng
recordid cdi_proquest_miscellaneous_2664791053
source Springer Link
subjects Biomedical and Life Sciences
Biomedicine
Brief Report
Capsicum annuum
Capsicum frutescens
Cloning
Flowers & plants
Genomes
Infections
Infectious Diseases
Laboratories
Medical Microbiology
Nicotiana benthamiana
Nucleotide sequence
p1 Protein
Pathogenicity
Plant virus diseases
Plant viruses
Potyvirus
Proteins
Virology
Viruses
Yellowing
title Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T11%3A55%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Construction%20of%20an%20infectious%20full-length%20and%20eGFP-tagged%20cDNA%20clone%20of%20a%20chilli%20ringspot%20virus%20isolate%20from%20Yunnan%20province,%20China&rft.jtitle=Archives%20of%20virology&rft.au=Hua,%20Mengying&rft.date=2022-07-01&rft.volume=167&rft.issue=7&rft.spage=1583&rft.epage=1587&rft.pages=1583-1587&rft.issn=0304-8608&rft.eissn=1432-8798&rft_id=info:doi/10.1007/s00705-022-05457-z&rft_dat=%3Cproquest_cross%3E2664791053%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c370t-e35a6bb84e002462d5fdd15c6c27161de6cd9871941786926781f73cc5de648f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2672174238&rft_id=info:pmid/35567696&rfr_iscdi=true