Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification

A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) via π-stacking interactions. Thi...

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Bibliographic Details
Published in:RSC advances 2018-09, Vol.8 (56), p.329-3215
Main Authors: Zhang, Jun, Ran, Fengying, Zhou, Wenbo, Shang, Bing, Yu, Fei, Wu, Lun, Hu, Wanbao, He, Xueqin, Chen, Qinhua
Format: Article
Language:English
Subjects:
Amplification
Chemistry
Fluorescence
Proteins
Saliva
Urine
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Summary:A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) via π-stacking interactions. This results in quenching of the fluorescent label and no fluorescence signal is observed. Upon adding MUC1, the probe sequences could be specifically recognized by MUC1, leading to an increase in the fluorescence intensity. The detection limit is as low as 10 pg mL −1 , and a linear range from 50 pg mL −1 to 100 ng mL −1 . The assay is specific and sensitive, and successfully applied to the determination of MUC1 in spiked human serum, urine and saliva. Importantly, the proposed aptasensing strategy has great potential in detecting various protein and even cancer cells. A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed.
ISSN:2046-2069
2046-2069
DOI:10.1039/c8ra06498a