Esophageal striated muscle hypertrophy and muscle fiber type transformation in MSTN knockout pigs

• Published in: Transgenic research 2022-06, Vol.31 (3), p.341-349
• Main Authors: Jin, Zhengyun, Choe, Hak Myong, Lv, Sitong, Chang, Shuangyan, Yin, Xijun
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Esophagus; Esophagus - metabolism; Genetic Engineering; Hogs; Hypertrophy; Hypertrophy - metabolism; Immunofluorescence; Life Sciences; Molecular Medicine; mRNA; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - metabolism; Musculoskeletal system; Myocytes; MyoD protein; Myogenin; Myosin; Myosin Heavy Chains - genetics; Myosin Heavy Chains - metabolism; Myostatin; Myostatin - genetics; Original Paper; Plant Genetics and Genomics; Protein expression; RNA, Messenger; Skeletal muscle; Smooth muscle; Swine - genetics; Transgenics
• ISSN: 0962-8819; 1573-9368
• DOI: 10.1007/s11248-022-00309-7

Myostatin (MSTN) is a member of the transforming growth factor-β superfamily that inhibits skeletal muscle growth and development. The esophagus is composed of skeletal muscle and smooth muscle, but the effect of MSTN on esophagus striated muscle (ESM) is unknown. The present study investigated the role of MSTN in ESM using MSTN mutant pigs through histological, gene and protein expression analysis in ESM of MSTN knockout ( MSTN −/− ) pigs and their wild type (WT) littermates. Hematoxylin–eosin staining showed that the fiber cross-sectional areas in ESM of MSTN −/− pigs were significantly larger than WT pigs ( P