Antifungal activity screening of fractions from Annona cherimola Mill. leaf extract against Fusarium oxysporum

The antifungal effect of ethanolic extract fractions of Annona cherimola leaves against the mycelial growth of Fusarium oxysporum was studied. The ethanolic crude extract was solvent partitioned and the ethyl acetate phase was fractionated by column or preparative thin-layer chromatography. All frac...

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Bibliographic Details
Published in:Archives of microbiology 2022-06, Vol.204 (6), p.330-330, Article 330
Main Authors: Méndez-Chávez, Manuel, Ledesma-Escobar, Carlos A., Hidalgo-Morales, Madeleine, Rodríguez-Jimenes, Guadalupe del Carmen, Robles-Olvera, Victor José
Format: Article
Language:English
Subjects:
Acetic acid
Annona cherimola
Antifungal activity
Biochemistry
Biological activity
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cherimoya
Column chromatography
Diffusion plating
Ecology
Ethyl acetate
Fungicides
Fusarium oxysporum
In vitro methods and tests
Leaves
Life Sciences
Microbial Ecology
Microbiology
Mycelia
Original Paper
Plant diseases
Plant extracts
Reagents
Screening
Thin layer chromatography
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Summary:The antifungal effect of ethanolic extract fractions of Annona cherimola leaves against the mycelial growth of Fusarium oxysporum was studied. The ethanolic crude extract was solvent partitioned and the ethyl acetate phase was fractionated by column or preparative thin-layer chromatography. All fractions were developed on TLC and analyzed for acetogenins (ACG) with Kedde reagent. The antifungal effect assays were carried out in vitro by the diffusion method on PDA plates. The ethanolic extract of A. cherimola leaves was highly active against F. oxysporum growth; subfractions obtained from the antifungal screening had a significant effect ( p  
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-02944-4