A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of plum viroid I (PlVd-I)

Plum viroid I (PlVd-I) is found in marbling and corky flesh diseased plum trees in South Africa. In this study a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the high-throughput detection of PlVd-I was developed. This assay can be performed on crude extracts and d...

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Bibliographic Details
Published in:Journal of virological methods 2022-08, Vol.306, p.114543-114543, Article 114543
Main Authors: Bester, Rachelle, Maree, Hans J.
Format: Article
Language:English
Subjects:
Colorimetric
Corky flesh
Marbling
Pospiviroidae
Real-time RT-LAMP
Stone fruit
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Summary:Plum viroid I (PlVd-I) is found in marbling and corky flesh diseased plum trees in South Africa. In this study a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the high-throughput detection of PlVd-I was developed. This assay can be performed on crude extracts and detection can either be a pH dependent colorimetric reaction or a real-time fluorescent signal reaction. The false discovery rate was shown to be low and no decrease in sensitivity was detected compared to RT-PCR. The RT-LAMP assay allows for the fast and cost-effective detection of PlVd-I that will curtail the distribution of infected plant material. •A one-step RT-LAMP assay was developed for the detection of plum viroid I (PlVd-I).•The RT-LAMP assays can be performed on crude extracts from leaf material.•Low false discovery rate and no decrease in sensitivity compared to RT-PCR.•Low-cost detection assay to limit the distribution of infected planting material.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2022.114543