Global DNA hypomethylation and the expression profile of DNA methyltransferase genes in late-onset neonatal sepsis

Infectious organisms tend to cause DNA methylation changes. Thus, this paper aims to study global DNA methylation and the expression of DNA methyltransferase (DNMT) genes in late-onset neonatal sepsis (LONS). Global and Alu DNA methylation and expression levels of DNMT were performed using 5mc ELISA...

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Bibliographic Details
Published in:Epigenomics 2022-06, Vol.14 (11), p.671-682
Main Authors: Sankar, Saranya, Maruthai, Kathirvel, Zachariah, Bobby, Bethou, Adhisivam
Format: Article
Language:English
Subjects:
5-mc ELISA
Alu repetitive element
DNA methylation
DNMT
Neonatal sepsis
ROC
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Summary:Infectious organisms tend to cause DNA methylation changes. Thus, this paper aims to study global DNA methylation and the expression of DNA methyltransferase (DNMT) genes in late-onset neonatal sepsis (LONS). Global and Alu DNA methylation and expression levels of DNMT were performed using 5mc ELISA, methylation-specific PCR and quantitative real-time-PCR, respectively for LONS and controls. Significant hypomethylation of global DNA and Alu DNA methylation and lower expression of and were observed in LONS compared with controls. Receiver operating characteristic analysis of global and Alu DNA methylation showed good discrimination for the identification of LONS. The hypomethylation of global DNA and Alu elements is evident in neonates with LONS. This may be clinically useful for the prognosis of LONS.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2022-0040