Loading…

Characterization of alkaloid profile of Hyoscyamus reticulatus L. and Atropa belladonna subsp. caucasica (Kreyer) Avet by LC-MS and NMR

The alkaloid profile of L. and subsp. (Kreyer) Avet have not been characterized yet. UHPLC-PDA-Q-TOF-MS/MS and LC-DAD-QqQ-MS/MS methods were used herein to characterize the metabolite profiles of these plants. Flash chromatography in combination with preparative- and semi preparative HPLC were utili...

Full description

Saved in:
Bibliographic Details
Published in:Natural product research 2023-10, Vol.37 (19), p.1-3362
Main Authors: Valiyeva, Adila, Felegyi-Tóth, Csenge Anna, Várnai, Bianka, Garaev, Eldar, Béni, Szabolcs, Kursinszki, László
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The alkaloid profile of L. and subsp. (Kreyer) Avet have not been characterized yet. UHPLC-PDA-Q-TOF-MS/MS and LC-DAD-QqQ-MS/MS methods were used herein to characterize the metabolite profiles of these plants. Flash chromatography in combination with preparative- and semi preparative HPLC were utilized for the isolation of the compounds of interest. The structure of the isolated compounds was proposed based on their MS/MS fragmentation and NMR characteristics. As a total of 19 tropane derivatives, two tyramine derivatives ( - - and - - feruloyl tyramine), a lignanamide (grossamide), an alkylamide (pellitorine) were identified in the root and herb extracts of . Moreover, rutin and caffeoylquinic acids were found in the leaves and fruits, while kaempferol-3- -glucoside-7- -rhamnoside and quercetin-3- -glucoside-rhamnoside-rhamnoside were exclusively characteristic for the leaves of . The root and herb extracts of contained 16 tropane alkaloid derivatives along with methyl tropate and two tyramine derivatives.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2022.2072841