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Characterization of alkaloid profile of Hyoscyamus reticulatus L. and Atropa belladonna subsp. caucasica (Kreyer) Avet by LC-MS and NMR
The alkaloid profile of L. and subsp. (Kreyer) Avet have not been characterized yet. UHPLC-PDA-Q-TOF-MS/MS and LC-DAD-QqQ-MS/MS methods were used herein to characterize the metabolite profiles of these plants. Flash chromatography in combination with preparative- and semi preparative HPLC were utili...
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Published in: | Natural product research 2023-10, Vol.37 (19), p.1-3362 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The alkaloid profile of
L. and
subsp.
(Kreyer) Avet have not been characterized yet. UHPLC-PDA-Q-TOF-MS/MS and LC-DAD-QqQ-MS/MS methods were used herein to characterize the metabolite profiles of these plants. Flash chromatography in combination with preparative- and semi preparative HPLC were utilized for the isolation of the compounds of interest. The structure of the isolated compounds was proposed based on their MS/MS fragmentation and NMR characteristics. As a total of 19 tropane derivatives, two tyramine derivatives (
-
- and
-
- feruloyl tyramine), a lignanamide (grossamide), an alkylamide (pellitorine) were identified in the root and herb extracts of
. Moreover, rutin and caffeoylquinic acids were found in the leaves and fruits, while kaempferol-3-
-glucoside-7-
-rhamnoside and quercetin-3-
-glucoside-rhamnoside-rhamnoside were exclusively characteristic for the leaves of
. The root and herb extracts of
contained 16 tropane alkaloid derivatives along with methyl tropate and two tyramine derivatives. |
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ISSN: | 1478-6419 1478-6427 |
DOI: | 10.1080/14786419.2022.2072841 |