Au–Se bonded nanoprobe for prostate specific antigen detection in serum

• Published in: Analytica chimica acta 2022-06, Vol.1210, p.339852-339852, Article 339852
• Main Authors: Gao, Xiaonan, Niu, Tianrun, Xia, Qinghua, Hu, Bo, Zhao, Zengteng, Feng, Xinyi, Yang, Jingqi, Tang, Bo, Xu, Kehua
• Format: Article
• Language: English
• Subjects: Au–Se bond; Biosensing Techniques - methods; Cancer diagnosis; Gold; Humans; Limit of Detection; Male; Metal Nanoparticles; Prostate specific antigen; Prostatic Neoplasms - diagnosis; Protein analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2022.339852

The detection of prostate specific antigen (PSA) in serum can realize early diagnosis of prostate cancer and prevent the occurrence of prostate tumors, as well as offering guidance during the therapy. Herein, a Au–Se bonded nanoprobes that can specifically detect PSA was designed and constructed. The peptide chains that can be specifically cleaved by PSA were firstly functionalized with fluorescent dye and selenol, and then bind to the Au nanoparticles to produce the probe. The dye's fluorescence was quenched due to the FRET effect, but recovered by PSA's cutting. The nanoprobe can detect PSA in serum with extraordinary anti-interference ability against other proteins (detection range 1–40 ng/mL). This work provides a new method for the detection of PSA in serum, and has potential guiding significance for clinical PSA detection. [Display omitted] •A prostate antigen-specific nanoprobe was constructed through the Au–Se bond, which can detect prostate antigen in serum with high fidelity, sensitivity and selectivity.•The detection concentration range of the nanoprobe for prostate antigen is 1–40 ng/mL, and the linear correlation coefficient is 0.996.•The nanoprobe is stable under physiological conditions, and can be applied to the detection of patients' serum samples.