Development of a system of heparin multilayers on titanium surfaces for dual growth factor release

The aim of the present study was to establish a modular platform of poly‐L‐lysine‐heparin (PLL‐Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growt...

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Published in:Journal of biomedical materials research. Part A 2022-09, Vol.110 (9), p.1599-1615
Main Authors: Behrens, Christina, Kauffmann, Philipp, Hahn, Nikolaus, Giesecke, Ariane, Schirmer, Uwe, Liefeith, Klaus, Schliephake, Henning
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Angiogenesis
Anticoagulants
biofunctionalization
Biomedical materials
bone morphogenic proteins
Cell proliferation
controlled release
Endothelial cells
Growth factors
Heparin
heparin polyelectrolyte multilayer
Lysine
Mesenchyme
Modular systems
Multilayers
Polyelectrolytes
Stem cells
Surface properties
Titanium
Umbilical vein
Vascular endothelial growth factor
Von Willebrand factor
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Summary:The aim of the present study was to establish a modular platform of poly‐L‐lysine‐heparin (PLL‐Hep) polyelectrolyte multilayer (PEM) coatings on titanium surfaces for dual growth factor delivery of recombinant human bone morphogenic protein 2 (rhBMP2) and recombinant human vascular endothelial growth factor 165 (rhVEGF165) in clinically relevant quantities. Release characteristics for both growth factors differed significantly depending on film architecture. rhBMP2 induced activation of alkaline phosphatase in C2C12 cells and proliferation of human mesenchymal stem cells (hMSCs). rhVEGF mediated induction of von Willebrand factor (vWF) in hMSCs and proliferation of human umbilical vein endothelial cells. Osteogenic and angiogenic effects were modified by variation in cross‐linking and architecture of the PEMs. By creating multilayer films with distinct zones, release characteristics and proportion of both growth factor delivery could be tuned and surface‐activity modified to enhance angiogenic or osteogenic function in various ways. In summary, the system provides a modular platform for growth factor delivery that allows for individual composition and accentuation of angiogenic and osteogenic surface properties.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.37411