Cultured and uncultured microbial community associated with biogas production in anaerobic digestion processes

• Published in: Archives of microbiology 2022-06, Vol.204 (6), p.340-340, Article 340
• Main Authors: Ottoni, Júlia Ronzella, Bernal, Suzan Prado Fernandes, Marteres, Tiago Joelzer, Luiz, Franciele Natividade, dos Santos, Viviane Piccin, Mari, Ângelo Gabriel, Somer, Juliana Gaio, de Oliveira, Valéria Maia, Passarini, Michel Rodrigo Zambrano
• Format: Article
• Language: English
• Subjects: Alternative energy sources; Anaerobic digestion; Anaerobic microorganisms; Anaerobic processes; Animal wastes; Biochemistry; Biogas; Biomedical and Life Sciences; Biotechnology; Cell Biology; Community involvement; Ecology; Firmicutes; Industrial plants; Inorganic carbon; Life Sciences; Lignocellulose; Livestock; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microorganisms; Organic acids; Original Paper; Refuse as fuel; Renewable energy sources; Renewable resources; Rhodococcus; rRNA 16S; Starter cultures; Substrates; Sugar; Sustainable development; Volatile solids
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-02819-8

The search for sustainable development has increased interest in the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock, and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly. The present study aimed to evaluate the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed the domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing of isolates recovered from the starter culture, the genera Rhodococcus (Actinobacteria phylum) , Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Proteiniclasticum (Firmicutes phylum) , and Luteimonas (Proteobacteria phylum) were identified, genera that were not observed in the metabarcoding data. The volatile solids, volatile organic acids, and total inorganic carbon reached 659.10 g kg −1 , 717.70 g kg −1 , 70,005.0 g kg −1 , respectively. The cultured groups are involved in the metabolism of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing, and culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated provide robust and comprehensive information about the microbial communities involved in processes of the production of biogas in anaerobic digestion processes.