Genotoxic assessment of cerium and magnesium nanoparticles and their ionic forms in Eisenia hortensis coelomocytes by alkaline comet assay

The present study aimed to evaluate the genotoxic potential of cerium oxide (CeO2), magnesium oxide (MgO) nanoparticles and their ionic forms by alkaline comet assay. Eisenia hortensis were exposed to different series of concentrations (25, 50, 100, 200, and 400 μg/ml) of chemicals for 48 h to find...

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Bibliographic Details
Published in:Microscopy research and technique 2022-09, Vol.85 (9), p.3095-3103
Main Authors: Güneş, Merve, Yalçın, Burçin, Ali, Muhammad Muddassir, Ciğerci, İbrahim Hakkı, Kaya, Bülent
Format: Article
Language:English
Subjects:
Bioassays
Cerium
cerium oxide
Cerium oxides
Coelomocytes
Comet assay
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
ecotoxicology
Eisenia hortensis
Electron microscopy
Genotoxicity
Magnesium
Magnesium oxide
Nanoparticles
Superconductors (materials)
Toxicity
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Summary:The present study aimed to evaluate the genotoxic potential of cerium oxide (CeO2), magnesium oxide (MgO) nanoparticles and their ionic forms by alkaline comet assay. Eisenia hortensis were exposed to different series of concentrations (25, 50, 100, 200, and 400 μg/ml) of chemicals for 48 h to find LC50. The LC50 for MgO and CeO2 NPs were 70 and 80 μg/ml. Whereas, the LC50 for their ionic forms were 50 and 70 μg/ml. To assess the potential DNA damage caused by the chosen chemicals, E. hortensis was further exposed for 48 h to the following concentrations, based on their respective LC50s: LC50/2, LC50, and 2xLC50. Comet scores demonstrated the significant increase (p 
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.24168