Monitoring, cross‐resistance, inheritance, and fitness costs of brown planthoppers, Nilaparvata lugens, resistance to pymetrozine in China

• Published in: Pest management science 2022-10, Vol.78 (10), p.3980-3987
• Main Authors: Song, Xin‐Yu, Peng, Yu‐Xuan, Wang, Li‐Xiang, Ye, Wen‐Nan, Pei, Xin‐Guo, Zhang, Yan‐Chao, Zhang, Shuai, Gao, Cong‐Fen, Wu, Shun‐Fan
• Format: Article
• Language: English
• Subjects: cross‐resistance; Fitness; fitness cost; Hatchability; heritability; Inheritances; insecticide resistance management; Insects; Monitoring; Nilaparvata lugens; Pattern analysis; Pest control; Pest resistance; Pesticide resistance; Pests; Polygenic inheritance; pymetrozine; Reproductive fitness; resistance monitoring
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.7017

BACKGROUND The brown planthopper, Nilaparvata lugens, is considered the most destructive pest of rice in many Asian countries including China. Use of pymetrozine in insect resistance management (IRM) has been one strategy to control this pest. In this study, we reported the status of pymetrozine resistance in Nilaparvata lugens (Stål) collected from China over the period 2017–2021 and selected a strain of N. lugens resistant to pymetrozine and evaluated the cross‐resistance, inheritance and fitness costs of the resistance. RESULTS Monitoring data (2017–2021) showed that field populations of N. lugens in China developed moderate‐ to high‐level pymetrozine resistance during these 5 years. By continuous selection with pymetrozine in the lab, the pymetrozine selected N. lugens strain (Pym‐R98) developed a 225.2‐fold resistance compared to a susceptible strain. The Pym‐R98 strain showed high cross‐resistance to dinotefuran (66.6‐fold) and low cross‐resistance to nitenpyram (5.2‐fold) and sulfoxaflor (5.8‐fold). Inheritance pattern analysis of Pym‐R93 revealed that resistance to pymetrozine was polygenic, autosomal and incompletely dominant. Fitness costs of pymetrozine resistance were present in Pym‐R90 and WA2020 strains with a relative fitness of 0.72 and 0.60, respectively. The developmental duration of Pym‐R90 and WA2020 was significantly longer and hatchability was significantly lower compared to pymetrozine‐susceptible strain (Pym‐S). CONCLUSIONS N. lugens has developed high level of resistance to pymetrozine. Pymetrozine‐resistance brown planthopper had cross‐resistance with some of neonicotinoids such as dinotefuran, nitenpyram and sulfoxaflor. The autosomal, incompletely dominant and polygenic resistance to pymetrozine in N. lugens and the fitness costs associated with this resistance can be exploited in IRM strategies to preserve the lifetime of pymetrozine for control of N. lugens in China. © 2022 Society of Chemical Industry. N. lugens has reached high resistance levels to pymetrozine. The pymetrozine‐resistant strain showed strong cross‐resistance to dinotefuran. The autosomal, incomplete dominant and polygenic inheritance pattern of resistance to pymetrozine in N. lugens was confirmed. Fitness costs of pymetrozine resistance were present in pymetrozine‐resistant strain.