Rotavirus incapable of NSP6 expression can cause diarrhea in suckling mice

The group A rotavirus (RVA) genome comprising 11 double-stranded RNAs encodes six structural proteins (VP1-VP4, VP6, and VP7) and six non-structural proteins (NSP1-NSP6). Among these 12 rotaviral proteins, NSP6 has been less studied as to its function. We previously prepared a recombinant NSP6-defic...

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Bibliographic Details
Published in:Journal of general virology 2022-05, Vol.103 (5)
Main Authors: Fukuda, Saori, Kugita, Masanori, Higashimoto, Yuki, Shiogama, Kazuya, Tsujikawa, Hanako, Moriguchi, Kyoko, Ito, Naoto, Sugiyama, Makoto, Nagao, Shizuko, Murata, Takayuki, Taniguchi, Koki, Komoto, Satoshi
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cells, Cultured
Diarrhea
Mice
Rotavirus - genetics
Rotavirus Infections
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Summary:The group A rotavirus (RVA) genome comprising 11 double-stranded RNAs encodes six structural proteins (VP1-VP4, VP6, and VP7) and six non-structural proteins (NSP1-NSP6). Among these 12 rotaviral proteins, NSP6 has been less studied as to its function. We previously prepared a recombinant NSP6-deficient RVA derived from simian strain SA11-L2 by reverse genetics, and found that the NSP6-deficient virus grew well in cell culture, although its growth was less abundant than that of the parental SA11-L2 strain. In this study, we examined the potency of a recombinant RVA incapable of NSP6 expression to cause diarrhoea in suckling mice. The suckling mice infected with the NSP6-deficient virus apparently experienced diarrhoea, although the symptom was milder and the duration of diarrhoea was shorter than in the mice infected with the authentic SA11-L2 strain. Thus, together with the results obtained for cultured cells in the previous study, it can be concluded that NSP6 is not necessarily required for replication and pathogenicity and .
ISSN:0022-1317
1465-2099
DOI:10.1099/jgv.0.001745