Lanthanum oxide nanoparticle-collagen bio matrix induced endothelial cell activation for sustained angiogenic response for biomaterial integration

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2022-08, Vol.216, p.112589-112589, Article 112589
• Main Authors: Vijayan, Vinu, Lakra, Rachita, Korrapati, Purna Sai, Kiran, Manikantan Syamala
• Format: Article
• Language: English
• Subjects: Angiogenesis; Biomaterial; Collagen; Lanthanum oxide; Tissue engineering
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2022.112589

Rare earth lanthanum oxide nanoparticle reinforced collagen biomatrix that elicited the endothelial cell activation to promote angiogenesis for biomaterial integration was developed and evaluated in the present study. The structural integrity of collagen was not compromised on crosslinking of lanthanum oxide nanoparticle to collagen biomolecule. As-synthesised collagen biomatrix was shown to have improved mechanical strength, a lesser susceptibility to proteolytic degradation and good swelling properties. Superior cytocompatibility, hemocompatibility and minimal ROS generation was observed with Lanthanum oxide nanoparticle reinforced collagen bio matrix. The Lanthanum oxide nanoparticle reinforced collagen bio matrix elicited endothelial cell activation eliciting pro-angiogensis as observed in tube formation and aortic arch assays. The bio-matrix promoted the infiltration and proliferation of endothelial cells which is an unexplored domain in the area of tissue engineering that is very essential for biomaterial integration into host tissue. The wound healing effect of Lanthanum oxide nanoparticle stabilized collagen showed enhanced cell migration in vitro in cells maintained in Lanthanum oxide nanoparticle reinforced collagen bio matrix. The study paves the way for developing rare earth-based dressing materials which promoted biomatrix integration by enhancing vascularisation for tissue regenerative applications in comparison with traditional biomaterials. [Display omitted] •Collagen based ECM mimicking scaffold strengthened with lanthanum oxide nanoparticles.•Enhanced mechanical, structural and biological stability compared to native collagen.•Remarkable Pro-angiogenic potency compared to native collagen scaffolds.•The scaffolds showed efficient wound healing ability in vitro.•Potential use of rare-earth based nanoparticles in advanced tissue repair process.