The Morphology, Ultrastructure and Molecular Phylogeny of a New Soil-Dwelling Kinetoplastid Avlakibodo gracilis gen. et sp. nov. (Neobodonida; Kinetoplastea)

• Published in: Protist 2022-08, Vol.173 (4), p.125885-125885, Article 125885
• Main Authors: Belyaev, Artem O., Zagumyonnyi, Dmitriy G., Mylnikov, Alexander P., Tikhonenkov, Denis V.
• Format: Article
• Language: English
• Subjects: 18S rRNA; Avlakibodo gracilis; heterotrophic flagellates; morphology; Neobodonida; ultrastructure
• ISSN: 1434-4610; 1618-0941
• DOI: 10.1016/j.protis.2022.125885

Kinetoplastids represent a stockpile of undiscovered protist diversity. Free-living members of this group have been studied less intensively compared to their important parasitic relatives. We have isolated a new soil-dwelling bacteriotrophic kinetoplastid, which is described here as a new genus and new species, Avlakibodo gracilis gen. et sp. nov. Phylogenetic analysis of 18S rRNA genes showed highly supported sister relationship of this protist with the clade uniting Neobodo borokensis, Neobodo curvifilus, Neobodo saliens, Actuariola framvarensis, some Neobodo designis isolates and several environmental sequences, with high statistical support. We have reconstructed the organization of the microtubular cytoskeleton of A. gracilis and determined the origins of the main bands of microtubules. Characteristic ultrastructural features include cytostome associated microtubules (FAS), cytopharynx associated additional microtubules (CMT), microtubular prism (nemadesm) and three microtubular roots (R1, R2 and R3).