Rapamycin ameliorates brain damage and maintains mitochondrial dynamic balance in diabetic rats subjected to middle cerebral artery occlusion

To investigate the effect of rapamycin on mitochondrial dynamic balance in diabetic rats subjected to cerebral ischemia-reperfusion injury. Male Sprague Dawley (SD) rats ( n  = 78) were treated with high fat diet combined with streptozotocin injection to construct diabetic model in rats. Transient m...

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Bibliographic Details
Published in:Metabolic brain disease 2023-02, Vol.38 (2), p.409-418
Main Authors: Hei, Changchun, Zhou, Yujia, Zhang, Chenyang, Gao, Furong, Cao, Meiling, Yuan, Shilin, Qin, Yixin, Li, P. Andy, Yang, Xiao
Format: Article
Language:English
Subjects:
Animals
Apoptosis Regulatory Proteins - metabolism
Biochemistry
Biomarkers
Biomedical and Life Sciences
Biomedicine
Brain
Brain - metabolism
Brain damage
Brain Injuries - complications
Brain injury
Brain Ischemia - metabolism
Cell death
Cerebral blood flow
Diabetes
Diabetes mellitus
Diabetes Mellitus, Experimental - metabolism
Dynamin
High fat diet
Hyperglycemia
Immunohistochemistry
Infarction, Middle Cerebral Artery - drug therapy
Ischemia
Male
Metabolic Diseases
Mitochondria
Mitochondrial Dynamics
Neurology
Neurosciences
Occlusion
Oncology
Original Article
Proteins
Rapamycin
Rats
Rats, Sprague-Dawley
Reperfusion
Reperfusion Injury - complications
Reperfusion Injury - drug therapy
Sirolimus - pharmacology
Sirolimus - therapeutic use
Sirtuin 3 - metabolism
Streptozocin
TOR protein
TOR Serine-Threonine Kinases - metabolism
Western blotting
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Summary:To investigate the effect of rapamycin on mitochondrial dynamic balance in diabetic rats subjected to cerebral ischemia-reperfusion injury. Male Sprague Dawley (SD) rats ( n  = 78) were treated with high fat diet combined with streptozotocin injection to construct diabetic model in rats. Transient middle cerebral artery occlusion (MCAO) of 2 hours was induced and the brains were harvested after 1 and 3 days of reperfusion. Rapamycin was injected intraperitoneally for 3 days prior to and immediately after operation, once a day. The neurological function was assessed, infarct volumes were measured and HE staining as well as immunohistochemistry were performed. The protein of hippocampus was extracted and Western blotting were performed to detect the levels of mTOR, mitochondrial dynamin related proteins (DRP1, p-DRP1, OPA1), SIRT3, and Nix/BNIP3L. Diabetic hyperglycemia worsened the neurological function performance ( p  
ISSN:0885-7490
1573-7365
DOI:10.1007/s11011-022-01020-6