Evaluating old truths: Final adult size in holometabolous insects is set by the end of larval development

• Published in: Journal of experimental zoology. Part B, Molecular and developmental evolution Molecular and developmental evolution, 2023-05, Vol.340 (3), p.270-276
• Main Authors: Hanna, Lisa, Lamouret, Tom, Poças, Gonçalo M., Mirth, Christen K., Moczek, Armin P., Nijhout, Frederik H., Abouheif, Ehab
• Format: Article
• Language: English
• Subjects: allometry; Animals; Ants; body mass; Body Size; Camponotus; Drosophila; Drosophila melanogaster; holometabolous insects; insect growth; Insecta - growth & development; Larva; Manduca; morphometrics; Onthophagus
• ISSN: 1552-5007; 1552-5015
• DOI: 10.1002/jez.b.23165

For centuries, it has been understood that the final size of adult holometabolous insects is determined by the end of the larval stage, and that once they transform to adults, holometabolous insects do not grow. Despite this, no previous study has directly tested these “old truths” across holometabolous insects. Here, we demonstrate that final adult size is set at the end of the last larval stage in species representing each of the four orders of holometabolous insects: the fruit fly Drosophila melanogaster (Diptera), the tobacco hornworm Manduca sexta (Lepidoptera), the dung beetle Onthophagus taurus (Coleoptera), and the Florida carpenter ant Camponotus floridanus (Hymenoptera). Furthermore, in both D. melanogaster and C. floridanus, we show that the size of adult individuals fluctuates but does not significantly change. Therefore, our study finally confirms these two basic assumptions in the biology of insects, which have for centuries served as the foundation for studies of insect growth, size, and allometry. Graphical summary of the progression of the larval, pupal, and adult stages of four holometabolous insects. In these insects, growth occurs during the larval instars. No growth occurs in the pupal and adult stages. Research Highlights Our research confirms an age‐old assumption in holometabolous insects: the body size of terminal larvae highly correlates with the body size of adults. Our research also confirms that, during the adult stage, insects fluctuate in size but do not undergo significant growth.