Synthesis and characterization of single‐walled carbon nanotube: Cyto‐genotoxicity in Allium cepa root tips and molecular docking studies

• Published in: Microscopy research and technique 2022-09, Vol.85 (9), p.3193-3206
• Main Authors: Ince Yardimci, Atike, Istifli, Erman Salih, Acikbas, Yaser, Liman, Recep, Yagmucukardes, Nesli, Yilmaz, Selahattin, Ciğerci, İbrahim Hakkı
• Format: Article
• Language: English
• Subjects: allium; Allium cepa; Anaphase; Bioassays; Carbon; Chemical synthesis; Chemical vapor deposition; Chromosome aberrations; comet; Crystal structure; Crystallinity; Cytotoxicity; Damage; Deoxyribonucleic acid; Distilled water; DNA; DNA damage; Electron microscopy; Electrostatic properties; Free energy; Genotoxicity; Gravimetric analysis; Grooves; Hydrophobicity; Light scattering; Meristems; Methyl methanesulfonate; Microscopy; Molecular docking; Nanoparticles; Nanotechnology; Nanotubes; Nucleotides; Photon correlation spectroscopy; Raman spectroscopy; Scanning electron microscopy; Single wall carbon nanotubes; single‐walled carbon nanotubes; Spectroscopy; Spectrum analysis; Toxicology; Transmission electron microscopy
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.24177

Herein, single‐walled carbon nanotubes (SWCNTs) were synthesized by the thermal chemical vapor deposition (CVD) method, and characterized by scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy (EDX), transmission electron microscopy (TEM), Raman spectroscopy, dynamic light scattering (DLS), and thermo‐gravimetric analysis (TGA). The results indicated that obtained nanotubes were SWCNTs with high crystallinity and their average diameter was 10.15 ± 3 nm. Allium cepa ana–telophase and comet assays on the root meristem were employed to evaluate the cytotoxic and genotoxic effects of SWCNTs by examining mitotic phases, mitotic index (MI), chromosomal aberrations (CAs), and DNA damage. A. cepa root tip cells were exposed to SWCNTs at concentrations of 12.5, 25, 50, and 100 μg/ml for 4 h. Distilled water and methyl methanesulfonate (MMS, 10 μg/ml) were used as the negative and positive control groups, respectively. It was observed that MIs decreased statistically significantly for all applied doses. Besides, CAs such as chromosome laggards, disturbed anaphase–telophase, stickiness and bridges and also DNA damage increased in the presence of SWCNTs in a concentration‐dependent manner. In the molecular docking study, the SWCNT were found to be a strong DNA major groove binder showing an energetically very favorable binding free energy of −21.27 kcal/mol. Furthermore, the SWCNT interacted effectively with the nucleotides on both strands of DNA primarily via hydrophobic π and electrostatic interactions. As a result, cytotoxic and genotoxic effects of SWCNTs in A. cepa root meristematic cells which is a reliable system for assessment of nanoparticle toxicology were demonstrated in this study. Research Highlights SWCNT synthesis with high crystallinity was achieved by the CVD method. Cytotoxic and genotoxic influences of SWCNTs were investigated. Allium and Comet tests were utilized. For all of the applied concentrations of SWCNTs, the MIs significantly decreased. SWCNTs were found genotoxic. Graphical designed for Cyto‐genetoxicity of SWCNTs in Allium cepa root tips and molecular docking studies.