Neutrophil extracellular traps induced by Porphyromonas gingivalis lipopolysaccharide modulate inflammatory responses via a Ca2+-dependent pathway

The aim of this study was to explore the effects and underlying mechanisms of Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) on the formation of neutrophil extracellular traps (NETs). NETs induced by 1 μg/ml P. gingivalis LPS were observed by a fluorescence microscope and quantifi...

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Bibliographic Details
Published in:Archives of oral biology 2022-09, Vol.141, p.105467-105467, Article 105467
Main Authors: Chen, Jia-lu, Tong, Yue, Zhu, Qin, Gao, Lan-qing, Sun, Ying
Format: Article
Language:English
Subjects:
Ca2
Lipopolysaccharide
Neutrophil extracellular traps
Porphyromonas gingivalis
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Summary:The aim of this study was to explore the effects and underlying mechanisms of Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) on the formation of neutrophil extracellular traps (NETs). NETs induced by 1 μg/ml P. gingivalis LPS were observed by a fluorescence microscope and quantified by a microplate reader. Quantities of extra- and intracellular P. gingivalis in neutrophils were determined to assay the bactericidal efficiency of NETs. Intracellular Ca2+ levels in neutrophils were explored by flow cytometry. Expressions of phospho-tumor progression locus 2 (p-TPL2), phospho-mitogen-activated protein kinase kinase1/2 (p-MEK1/2), phospho-extracellular signal-regulated kinase1/2 (p-ERK1/2), ORAI1, ORAI2 and peptidylarginine deiminase 4 (PAD4) were detected by Western blot. In addition, neutrophil elastase activities in NETs incubated with macrophages were assayed to evaluate their clearance. P. gingivalis LPS contributed to the formation of NETs and the increased levels of extracellular DNA (p 
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2022.105467