A “signal-off” electrochemiluminescence biosensing platform based on high efficiency quenching effect of functionalized copper oxide toward glutathione-gold nanoclusters

A “signal-off” Electrochemiluminescence (ECL) biosensing platform based on Glutathione-Au nanoclusters covered reduced graphene oxide (GSH-Au NCs@rGO) and Au nanoparticles functionalized copper oxide (Au@CuO) was fabricated. The GSH ligand protected Au NCs were spontaneously adsorbed on the rGO surf...

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Bibliographic Details
Published in:Talanta (Oxford) 2022-11, Vol.249, p.123649-123649, Article 123649
Main Authors: Dong, Hui, Wu, Zhanglei, Liu, Shanghua, Li, Yueyuan, Jiang, Feng, Liu, Qing, Wang, Ping, Xu, Zhen, Li, Yueyun
Format: Article
Language:English
Subjects:
Cardiac troponin I
Electrochemiluminescence
Functionalized copper oxide
Glutathione-Au nanoclusters
Quenching effect
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Summary:A “signal-off” Electrochemiluminescence (ECL) biosensing platform based on Glutathione-Au nanoclusters covered reduced graphene oxide (GSH-Au NCs@rGO) and Au nanoparticles functionalized copper oxide (Au@CuO) was fabricated. The GSH ligand protected Au NCs were spontaneously adsorbed on the rGO surface via Van der Waals force. As ECL emitters, GSH-Au NCs@rGO not only support more luminophores and immobilization of bioreceptor units also facilitates mass transfer, accelerating ECL excitation to obtain a higher ECL signal intensity. Remarkably, Au@CuO with good biocompatibility was first applied as a quenching probe. Au@CuO (acceptor)-dependent resonance energy transfers (RET) with GSH-Au NCs@rGO (donor) could effectively quenched the ECL intensity to a reasonable range for requirements of trace analysis. The proposed ECL biosensing platform was evaluated with cardiac troponin I (cTnI) as a model analyte, achieving a low detection limit of 54.95 fg/mL. This strategy may provide as new approaches for the sensitive detection of biomarkers in the early clinical diagnosis of diseases. [Display omitted] •GSH-Au NCs@rGO as ECL emitter could generate a stable and high-intensity ECL signal.•Au@CuO was firstly applied as a quenching prober of ECL signal.•The biosensor displayed low detection limit of 54.95 fg/mL for cTnI detection.•The actual sample analysis confirmed the excellent analytical capabilities of the biosensor.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2022.123649