DNA walker-powered ratiometric SERS cytosensor of circulating tumor cells with single-cell sensitivity

• Published in: Biosensors & bioelectronics 2022-10, Vol.213, p.114442-114442, Article 114442
• Main Authors: Xiong, Jingrong, Dong, Chen, Zhang, Jingjing, Fang, Xinyue, Ni, Jie, Gan, Hongyu, Li, Jinxiang, Song, Chunyuan
• Format: Article
• Language: English
• Subjects: Aptamer; Circulating tumor cells; DNA walker; DNAzyme; Nanostructure; Ratiometric surface-enhanced Raman spectroscopy
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2022.114442

Identification and detection of extreme rare circulating tumor cells (CTCs) in peripheral blood can precisely monitor cancer recurrence and metastasis, however, how to ultra-sensitively and reliably detect CTCs is a big challenge. In this work, a ratiometric surface-enhanced Raman spectroscopy (SERS)-based strategy for ultra-sensitively and nondestructively detecting CTCs was proposed via CTCs-triggered DNA walker-assisted assembly of plasmonic nanostructure networks consisting of Walker probes and SERS tags. The Walker probes were prepared by modifying Fe3O4@SiO2@Au nanoparticles (GMNPs) with ROX-labeled EpCAM aptamer-blocked Zn2+-specific DNAzyme and hairpin-structured single-stranded DNAs H1, and the SERS tags were constructed by co-labelling hairpin-structured single-stranded DNAs H2 and Raman molecules (DTNB) on Au NPs. The aptamers can recognize EpCAM-positive CTCs via the specific binding to EpCAM, so that the activity of DNAzymes is activated with the assistance of Zn2+ to launch the DNA walker to move around for the cleavage of H1 on GMNPs. The residual fragments of H1 on GMNPs can hybridize with H2 on SERS tags and result in the formation of Walker probe-SERS tag network nanostructures (Nw NSs) with rich SERS hot spots. The reliable SERS detection of CTCs is achieved by the stable ratiometric SERS signals of DTNB and ROX generated from the Nw NSs, and a good linear relation between ratiometric SERS signal and MCF-7 cells concentration was obtained with the detection limit low to 1 cell/mL. The recovery rate of MCF-7 cells in peripheral blood is in the range of 94.0%–104.5%, which indicates a good application prospect of the novel ratiometric SERS cytosensor in the clinic detection of EpCAM-positive CTCs.