Genome characterization and pathogenicity of two new Hyptis pectinata viruses transmitted by distinct insect vectors

Two newly described viruses belonging to distinct families, Rhabdoviridae and Geminiviridae, were discovered co-infecting Hyptis pectinata from a tropical dry forest of Ecuador. The negative-sense RNA genome of the rhabdovirus, tentatively named Hyptis latent virus (HpLV), comprises 13,765 nucleotid...

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Bibliographic Details
Published in:Phytopathology 2022-11, Vol.112 (11), p.2440-2448
Main Authors: Reyes-Proaño, Edison, Alvarez-Quinto, Robert, Delgado Jiménez, José A, Cornejo-Franco, Juan F, Mollov, Dimitre, Bejerman, Nicolas, Quito-Avila, Diego
Format: Article
Language:English
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Summary:Two newly described viruses belonging to distinct families, Rhabdoviridae and Geminiviridae, were discovered co-infecting Hyptis pectinata from a tropical dry forest of Ecuador. The negative-sense RNA genome of the rhabdovirus, tentatively named Hyptis latent virus (HpLV), comprises 13,765 nucleotides with seven open reading frames (ORFs) separated by the conserved intergenic region 3'-AAUUAUUUUGAU-5'. Sequence analyses showed identities as high as 56% for the polymerase, and 38% for the nucleocapsid, to members of the genus Cytorhabdovirus. Efficient transmission of HpLV was mediated by the pea aphid (Acyrthosiphon pisum) in a persistent replicative manner. The single-stranded DNA genome of the virus tentatively named Hyptis golden mosaic virus (HpGMV), shared homology with members of the genus Begomovirus with bipartite genomes. The DNA-A component consists of 2,716 nt, whereas the DNA-B component contains 2,666 nt. Pairwise alignments using the complete genomic sequence of DNA-A of HpGMV and closest relatives showed identities below the cutoff (
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-04-22-0130-R