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Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry
LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily...
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| Published in: | Analyst (London) 2022-06, Vol.147 (13), p.311-318 |
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| creator | Gao, Tianqi Lott, Aneirin A Huang, Fanran Rohokale, Rajendra Li, Qingjiang Olivos, Hernando J Chen, Sixue Guo, Zhongwu |
| description | LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily separated and identified using liquid chromatography (LC)-MS. In addition, like gangliosides, homologous lipid forms of GalNAc-LcGg4 showed the same fragmentation pattern, except for a uniform shift of their glycolipid product ions by a certain
m
/
z
number determined by the varied lipid structure. It was also disclosed that LcGg4 and its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, which are different only in the C4-configuration of their non-reducing end sugar residues, gave the same MS/MS product ions in similar relative intensities, as well as the same LC retention time, suggesting the challenge to differentiate epimeric GSLs by LC-MS. However, ion mobility spectrometry (IMS)-MS was able to efficiently separate and distinguish these epimers. This study has demonstrated the promise of IMS-MS for isomeric GSL characterization and the IMS-MS and LC-MS/MS combination for natural GSL analysis.
LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). |
| doi_str_mv | 10.1039/d2an00224h |
| format | article |
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m
/
z
number determined by the varied lipid structure. It was also disclosed that LcGg4 and its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, which are different only in the C4-configuration of their non-reducing end sugar residues, gave the same MS/MS product ions in similar relative intensities, as well as the same LC retention time, suggesting the challenge to differentiate epimeric GSLs by LC-MS. However, ion mobility spectrometry (IMS)-MS was able to efficiently separate and distinguish these epimers. This study has demonstrated the promise of IMS-MS for isomeric GSL characterization and the IMS-MS and LC-MS/MS combination for natural GSL analysis.
LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS).</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d2an00224h</identifier><identifier>PMID: 35695136</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Antigens ; Chromatography, Liquid - methods ; Gangliosides ; Homology ; Ion Mobility Spectrometry ; Ionic mobility ; Ions ; Lipids ; Liquid chromatography ; Mass spectrometry ; Neutral Glycosphingolipids ; Scientific imaging ; Spectroscopy ; Structural analysis ; Tandem Mass Spectrometry</subject><ispartof>Analyst (London), 2022-06, Vol.147 (13), p.311-318</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343h-6ce88c5900fb21c9737c4de17b38e0ee71f6b0473b0bdfa702f4a4d32325fa623</citedby><cites>FETCH-LOGICAL-c343h-6ce88c5900fb21c9737c4de17b38e0ee71f6b0473b0bdfa702f4a4d32325fa623</cites><orcidid>0000-0002-6690-7612 ; 0000-0002-0424-5836 ; 0000-0001-5302-6456</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35695136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Tianqi</creatorcontrib><creatorcontrib>Lott, Aneirin A</creatorcontrib><creatorcontrib>Huang, Fanran</creatorcontrib><creatorcontrib>Rohokale, Rajendra</creatorcontrib><creatorcontrib>Li, Qingjiang</creatorcontrib><creatorcontrib>Olivos, Hernando J</creatorcontrib><creatorcontrib>Chen, Sixue</creatorcontrib><creatorcontrib>Guo, Zhongwu</creatorcontrib><title>Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily separated and identified using liquid chromatography (LC)-MS. In addition, like gangliosides, homologous lipid forms of GalNAc-LcGg4 showed the same fragmentation pattern, except for a uniform shift of their glycolipid product ions by a certain
m
/
z
number determined by the varied lipid structure. It was also disclosed that LcGg4 and its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, which are different only in the C4-configuration of their non-reducing end sugar residues, gave the same MS/MS product ions in similar relative intensities, as well as the same LC retention time, suggesting the challenge to differentiate epimeric GSLs by LC-MS. However, ion mobility spectrometry (IMS)-MS was able to efficiently separate and distinguish these epimers. This study has demonstrated the promise of IMS-MS for isomeric GSL characterization and the IMS-MS and LC-MS/MS combination for natural GSL analysis.
LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS).</description><subject>Antigens</subject><subject>Chromatography, Liquid - methods</subject><subject>Gangliosides</subject><subject>Homology</subject><subject>Ion Mobility Spectrometry</subject><subject>Ionic mobility</subject><subject>Ions</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Neutral Glycosphingolipids</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Structural analysis</subject><subject>Tandem Mass Spectrometry</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkk1v1DAQhi0Eokvhwh0UiQtCCjj-SnxBqlpokVZwAM6W44w3rpI42A5S-BP9y3i7ZaEcLMszz7ya8TsIPa_w2wpT-a4jesKYENY_QJuKClZyTpqHaIMxpiURnJ2gJzFe52eFOX6MTigXkmdyg26-prCYtAQ9FKbXQZsEwf3Syfmp0FOXjx7W6GLhbdE5ayHAlAqY3QjhNjjBkvbVu2E1Ps69m3Z-cLPriq253LGiXYu91uhbN7i0FnEGk4IfIYW1HHWM9yJP0SOrhwjP7u5T9P3jh2_nV-X2y-Wn87NtaSijfSkMNI3hEmPbksrImtaGdVDVLW0AA9SVFS1mNW1x21ldY2KZZh0llHCrBaGn6P1Bd17aETqTh8pDqDm4UYdVee3U_czkerXzP5VkDW0YywKv7wSC_7FATGp00cAw6An8EhURNZcNE4Jm9NV_6LVfQv7XPdVgWUvRyEy9OVAm-BgD2GMzFVZ7n9UFOft86_NVhl_-2_4R_WNsBl4cgBDNMft3Uehvp6ax4w</recordid><startdate>20220627</startdate><enddate>20220627</enddate><creator>Gao, Tianqi</creator><creator>Lott, Aneirin A</creator><creator>Huang, Fanran</creator><creator>Rohokale, Rajendra</creator><creator>Li, Qingjiang</creator><creator>Olivos, Hernando J</creator><creator>Chen, Sixue</creator><creator>Guo, Zhongwu</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6690-7612</orcidid><orcidid>https://orcid.org/0000-0002-0424-5836</orcidid><orcidid>https://orcid.org/0000-0001-5302-6456</orcidid></search><sort><creationdate>20220627</creationdate><title>Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry</title><author>Gao, Tianqi ; Lott, Aneirin A ; Huang, Fanran ; Rohokale, Rajendra ; Li, Qingjiang ; Olivos, Hernando J ; Chen, Sixue ; Guo, Zhongwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343h-6ce88c5900fb21c9737c4de17b38e0ee71f6b0473b0bdfa702f4a4d32325fa623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antigens</topic><topic>Chromatography, Liquid - methods</topic><topic>Gangliosides</topic><topic>Homology</topic><topic>Ion Mobility Spectrometry</topic><topic>Ionic mobility</topic><topic>Ions</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Neutral Glycosphingolipids</topic><topic>Scientific imaging</topic><topic>Spectroscopy</topic><topic>Structural analysis</topic><topic>Tandem Mass Spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gao, Tianqi</creatorcontrib><creatorcontrib>Lott, Aneirin A</creatorcontrib><creatorcontrib>Huang, Fanran</creatorcontrib><creatorcontrib>Rohokale, Rajendra</creatorcontrib><creatorcontrib>Li, Qingjiang</creatorcontrib><creatorcontrib>Olivos, Hernando J</creatorcontrib><creatorcontrib>Chen, Sixue</creatorcontrib><creatorcontrib>Guo, Zhongwu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Tianqi</au><au>Lott, Aneirin A</au><au>Huang, Fanran</au><au>Rohokale, Rajendra</au><au>Li, Qingjiang</au><au>Olivos, Hernando J</au><au>Chen, Sixue</au><au>Guo, Zhongwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2022-06-27</date><risdate>2022</risdate><volume>147</volume><issue>13</issue><spage>311</spage><epage>318</epage><pages>311-318</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS). It was found that different forms of GalNAc-LcGg4 carrying homologous (d16:1/18:0) and (d18:1/18:0) lipids were easily separated and identified using liquid chromatography (LC)-MS. In addition, like gangliosides, homologous lipid forms of GalNAc-LcGg4 showed the same fragmentation pattern, except for a uniform shift of their glycolipid product ions by a certain
m
/
z
number determined by the varied lipid structure. It was also disclosed that LcGg4 and its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, which are different only in the C4-configuration of their non-reducing end sugar residues, gave the same MS/MS product ions in similar relative intensities, as well as the same LC retention time, suggesting the challenge to differentiate epimeric GSLs by LC-MS. However, ion mobility spectrometry (IMS)-MS was able to efficiently separate and distinguish these epimers. This study has demonstrated the promise of IMS-MS for isomeric GSL characterization and the IMS-MS and LC-MS/MS combination for natural GSL analysis.
LcGg4, a neutral glycosphingolipid (GSL) and cancer antigen, its epimers GalNAc-LcGg4 and GlcNAc-LcGg4, and three lipid forms of GalNAc-LcGg4 were studied by mass spectrometry (MS).</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>35695136</pmid><doi>10.1039/d2an00224h</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6690-7612</orcidid><orcidid>https://orcid.org/0000-0002-0424-5836</orcidid><orcidid>https://orcid.org/0000-0001-5302-6456</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Analyst (London), 2022-06, Vol.147 (13), p.311-318 |
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| source | Royal Society of Chemistry Journals |
| subjects | Antigens Chromatography, Liquid - methods Gangliosides Homology Ion Mobility Spectrometry Ionic mobility Ions Lipids Liquid chromatography Mass spectrometry Neutral Glycosphingolipids Scientific imaging Spectroscopy Structural analysis Tandem Mass Spectrometry |
| title | Structural characterization and analysis of different epimers of neutral glycosphingolipid LcGg4 by ion mobility spectrometry-mass spectrometry |
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