Cellular immunophenotype of major spine surgery in adults

Purpose ASD reconstructions are a major, sterile traumatic insult, likely causing perturbations to the immune systems. The immune response to surgery is associated with outcomes. The purpose of this study was to examine for a detectable immune signature associated with ASD surgery. Methods Consecuti...

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Bibliographic Details
Published in:Spine deformity 2022-11, Vol.10 (6), p.1375-1384
Main Authors: Turnbull, Isaiah R., Hess, Annie, Fuchs, Anja, Frazier, Elfaridah P., Ghosh, Sarbani, Hughes, Shin-Wen, Kelly, Michael P.
Format: Article
Language:English
Subjects:
Adult
Case Series
Flow Cytometry
HLA-DR Antigens - metabolism
Humans
Immunophenotyping
Medicine
Medicine & Public Health
Orthopedics
Programmed Cell Death 1 Receptor
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Summary:Purpose ASD reconstructions are a major, sterile traumatic insult, likely causing perturbations to the immune systems. The immune response to surgery is associated with outcomes. The purpose of this study was to examine for a detectable immune signature associated with ASD surgery. Methods Consecutive patients undergoing ASD surgery were approached and enrolled. Peripheral blood was drawn before incision, 4 h after, and 24 h after incision. Blood was stabilized and comprehensive flow cytometric immunophenotyping performed. Leukocyte population frequency, absolute number and activation marker expression were defined. Immunologic features were defined and analyzed by hierarchical clustering and principal component analysis (PCA). Changes over time were evaluated by repeated measures ANOVA (RMANOVA) and were corrected for a 1% false discovery rate. Post hoc testing was by Dunn’s test. p values of 
ISSN:2212-134X
2212-1358
DOI:10.1007/s43390-022-00524-6