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Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats
Previous studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therap...
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| Published in: | Inflammation 2022-12, Vol.45 (6), p.2243-2255 |
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| Main Authors: | , , , , , , , , , , , |
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| container_end_page | 2255 |
| container_issue | 6 |
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| container_title | Inflammation |
| container_volume | 45 |
| creator | Correia, Aristides Tadeu de Almeida, Francine Maria Augusto-Cottet, Marcia Cristina Nolasco, Patrícia Bento, Afonso Silva Alves Hirano, Hugo Kenji Matsushima de Souza, Maria Cecília Ribeiro dos Santos, Elizabete Silva de Castro, Júlia Helena Rodrigues Matsuda, Monique Pêgo-Fernandes, Paulo Manuel Pazetti, Rogerio |
| description | Previous studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (
p
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| doi_str_mv | 10.1007/s10753-022-01687-0 |
| format | article |
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p
< 0.05). Neutral mucin percentage was higher in Triple group (
p
< 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (
p
< 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (
p
< 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (
p
< 0.05). The number of polymorphonuclear cells was higher in the Triple group (
p
< 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (
p
< 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (
p
< 0.05). BSX did not change the mucociliary apparatus of rats.]]></description><identifier>ISSN: 0360-3997</identifier><identifier>EISSN: 1573-2576</identifier><identifier>DOI: 10.1007/s10753-022-01687-0</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cell number ; Gene expression ; Immunology ; Immunosuppressive agents ; Inflammation ; Interleukin 6 ; Internal Medicine ; Leukocytes (mononuclear) ; Leukocytes (neutrophilic) ; Leukocytes (polymorphonuclear) ; Lymphocytes ; Monoclonal antibodies ; MUC5B gene ; Mucin ; Original Article ; Pathology ; Pharmacology/Toxicology ; Respiratory tract ; Rheumatology</subject><ispartof>Inflammation, 2022-12, Vol.45 (6), p.2243-2255</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c303t-cf5f15eaeaf9866427627205afc72bca57f8fdfa5364b796209e0968e8b677503</cites><orcidid>0000-0002-1195-6732 ; 0000-0001-9755-5034</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Correia, Aristides Tadeu</creatorcontrib><creatorcontrib>de Almeida, Francine Maria</creatorcontrib><creatorcontrib>Augusto-Cottet, Marcia Cristina</creatorcontrib><creatorcontrib>Nolasco, Patrícia</creatorcontrib><creatorcontrib>Bento, Afonso Silva Alves</creatorcontrib><creatorcontrib>Hirano, Hugo Kenji Matsushima</creatorcontrib><creatorcontrib>de Souza, Maria Cecília Ribeiro</creatorcontrib><creatorcontrib>dos Santos, Elizabete Silva</creatorcontrib><creatorcontrib>de Castro, Júlia Helena Rodrigues</creatorcontrib><creatorcontrib>Matsuda, Monique</creatorcontrib><creatorcontrib>Pêgo-Fernandes, Paulo Manuel</creatorcontrib><creatorcontrib>Pazetti, Rogerio</creatorcontrib><title>Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats</title><title>Inflammation</title><addtitle>Inflammation</addtitle><description><![CDATA[Previous studies have shown that immunosuppressive drugs impair the airway mucociliary clearance of rats. However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (
p
< 0.05). Neutral mucin percentage was higher in Triple group (
p
< 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (
p
< 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (
p
< 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (
p
< 0.05). The number of polymorphonuclear cells was higher in the Triple group (
p
< 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (
p
< 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (
p
< 0.05). BSX did not change the mucociliary apparatus of rats.]]></description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell number</subject><subject>Gene expression</subject><subject>Immunology</subject><subject>Immunosuppressive agents</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Internal Medicine</subject><subject>Leukocytes (mononuclear)</subject><subject>Leukocytes (neutrophilic)</subject><subject>Leukocytes (polymorphonuclear)</subject><subject>Lymphocytes</subject><subject>Monoclonal antibodies</subject><subject>MUC5B gene</subject><subject>Mucin</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Pharmacology/Toxicology</subject><subject>Respiratory tract</subject><subject>Rheumatology</subject><issn>0360-3997</issn><issn>1573-2576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEYRYMoWKt_wFXAjZvoN8nktbPWV6EqiK5DJiYyZdrUZAbtv3fqCIILV3dz7uVyEDou4KwAkOe5AMkZAUoJFEJJAjtoVHDJCOVS7KIRMAGEaS330UHOCwBQWrERuri0uW7qz3ppK3wVfcYPscWz5drWCU_q9GE3-L5z0fWQTRs8bbxNduU8jgE_2TYfor1gm-yPfnKMXm6un6d3ZP54O5tO5sQxYC1xgYeCe-tt0EqIkkpBJQVug5O0cpbLoMJrsJyJspJaUNAetFBeVUJKDmyMTofddYrvnc-tWdbZ-aaxKx-7bKiQqqQlF6JHT_6gi9ilVf_OUMnKknGtWU_RgXIp5px8MOvUW0gbU4DZSjWDVNNLNd9SzfYFG0q5h1dvPv1O_9P6Ar2Qd_8</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Correia, Aristides Tadeu</creator><creator>de Almeida, Francine Maria</creator><creator>Augusto-Cottet, Marcia Cristina</creator><creator>Nolasco, Patrícia</creator><creator>Bento, Afonso Silva Alves</creator><creator>Hirano, Hugo Kenji Matsushima</creator><creator>de Souza, Maria Cecília Ribeiro</creator><creator>dos Santos, Elizabete Silva</creator><creator>de Castro, Júlia Helena Rodrigues</creator><creator>Matsuda, Monique</creator><creator>Pêgo-Fernandes, Paulo Manuel</creator><creator>Pazetti, Rogerio</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1195-6732</orcidid><orcidid>https://orcid.org/0000-0001-9755-5034</orcidid></search><sort><creationdate>20221201</creationdate><title>Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats</title><author>Correia, Aristides Tadeu ; 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However, considering the high specificity of basiliximab (BSX) and the absence of studies reporting its side effects, our aim was to investigate whether BSX, associated or not with triple therapy, impairs the mucociliary system. Forty rats were divided into 4 groups: Control, BSX, Triple, and BSX + Triple. After 15 days of treatment, animals were euthanized and the ciliary beating frequency (CBF), mucociliary transport velocity (MCTV), neutral and acid mucin production, Muc5ac and Muc5b gene expression, inflammatory cell number, and interleukin (IL)-6 concentration were analyzed. CBF and MCTV were lower in Triple and BSX + Triple groups (
p
< 0.05). Neutral mucin percentage was higher in Triple group (
p
< 0.05), and acid mucin percentage was higher in Triple and BSX + Triple groups (
p
< 0.05). The Muc5ac and Muc5b gene expression was higher in Triple and BSX + Triple groups (
p
< 0.05). Animals from Triple and BSX + Triple groups presented fewer mononuclear cells (
p
< 0.05). The number of polymorphonuclear cells was higher in the Triple group (
p
< 0.05). In the analysis of inflammatory cells in the blood, there was a decrease in lymphocytes and an increase in neutrophils in the Triple and BSX + Triple groups (
p
< 0.05). The concentration of IL-6 significantly increased in the animals of the Triple and BSX + Triple groups (
p
< 0.05). BSX did not change the mucociliary apparatus of rats.]]></abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s10753-022-01687-0</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-1195-6732</orcidid><orcidid>https://orcid.org/0000-0001-9755-5034</orcidid></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Cell number Gene expression Immunology Immunosuppressive agents Inflammation Interleukin 6 Internal Medicine Leukocytes (mononuclear) Leukocytes (neutrophilic) Leukocytes (polymorphonuclear) Lymphocytes Monoclonal antibodies MUC5B gene Mucin Original Article Pathology Pharmacology/Toxicology Respiratory tract Rheumatology |
| title | Basiliximab Does Not Impair Airway Mucociliary Clearance of Rats |
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