Hydroalcoholic extract of Remijia ferruginea accelerates the closure of skin wounds by modulating tissue morphology and antioxidant profile: An in vitro and in vivo study

• Published in: Journal of ethnopharmacology 2022-10, Vol.296, p.115464-115464, Article 115464
• Main Authors: Sarandy, Mariáurea Matias, Gusmão, Leandro José, Purgato, Gislaine Aparecida, Píccolo, Mayra Soares, Pinto da Matta, Sérgio Luis, Pizziolo, Virgínia Ramos, Diaz-Munoz, Gaspar, Nogueira Diaz, Marisa Alves
• Format: Article
• Language: English
• Subjects: Antioxidants defenses; Cells proliferation; Flavonoids; Herbal medicine; Wound heling
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2022.115464

Remijia ferruginea DC. (Rubiaceae) (syn. Cinchona ferruginea A.St.-Hil.) is used in traditional medicine for the treatment of wounds, fever and malaria. This study investigated in vitro the proliferative and antioxidant effects of hydroalcoholic extract of leaves of R. ferruginea (HERF) and in vivo the healing effect of ointment based on HERF. The plant extract was characterized by liquid chromatography/mass spectrometry. Cell proliferation assays and in vitro antioxidant activity were performed. In in vivo assays, wound contraction ax was evaluated, as well as histological analyzes such as cellularity, proportion of blood vessels and collagen type I and III index. In addition, analyzes of the antioxidant enzymes SOD, CAT and GST were performed. Our results showed in the chromatographic analysis that catechin, rutin and quercetin were the main phenolic compounds in the plant extract and may be responsible for the antioxidant and proliferative effects (p